12型腺病毒E1A基因转化的细胞对二酰基甘油介导的细胞杀伤具有高敏感性。

The high sensitivity of cells transformed by E1A gene of adenovirus type 12 to diacylglycerol-mediated cell killing.

作者信息

Shimura H, Matsuzaki A, Shiroki K, Ohtsu M, Fujinaga K, Onodera K, Kimura G

机构信息

Department of Virology, Kyushu University, Fukuoka, Japan.

出版信息

Cancer Lett. 1989 Feb;44(2):143-9. doi: 10.1016/0304-3835(89)90009-8.

DOI:10.1016/0304-3835(89)90009-8

PMID:2920374

Abstract

Viability of rat 3Y1 fibroblasts transformed by adenovirus type 12 (Ad12) was markedly impaired by the administration of dilinoleoylglycerol (DLG) to the culture medium. To identify the gene(s) of Ad12 responsible for the high sensitivity to DLG, we established several transformed sublines of 3Y1 induced by the viral E1A gene or by the mutants of Ad12 which have mutations in the E1A region. All of the transformed sublines of 3Y1 expressing either the 12S or the 13S message from the E1A region were highly sensitive to the cytotoxicity of DLG. We propose that the high sensitivity of Ad12-transformed cells to the DLG mediated cytotoxicity is attributable to the common function of E1A-12S and E1A-13S mRNA products.

摘要

向培养基中添加二亚油酰甘油（DLG）可显著损害由12型腺病毒（Ad12）转化的大鼠3Y1成纤维细胞的活力。为了鉴定Ad12中对DLG高度敏感负责的基因，我们建立了几个由病毒E1A基因或E1A区域有突变的Ad12突变体诱导的3Y1转化亚系。所有表达来自E1A区域的12S或13S信息的3Y1转化亚系对DLG的细胞毒性都高度敏感。我们提出，Ad12转化细胞对DLG介导的细胞毒性的高度敏感性归因于E1A - 12S和E1A - 13S mRNA产物的共同功能。