Lichtner R B, Erkell L J, Schirrmacher V, Nicolson G L
Department of Immunology and Genetics, German Cancer Research Center, Heidelberg.
Clin Exp Metastasis. 1989 Mar-Apr;7(2):175-86. doi: 10.1007/BF01787022.
The pyrimido-pyrimidine analogue RA 233 has pleiotropic and differential effects on cultured tumor cell clones isolated from the 13762NF rat mammary adenocarcinoma. A nonresponsive clone of low metastatic potential (MTC) was not modified in its cell fragility or invasive, adhesive or lung-colonizing properties by RA 233 treatment. In contrast, a drug-responsive clone of high metastatic potential (MTLn3) was rendered less invasive and its cell fragility was decreased with RA 233 treatment, although its adhesiveness to lung microvascular endothelial cells and subendothelial matrix was unaffected by RA 233. Lung colonization of intravenously injected MTLn3 cells in syngeneic rats was significantly increased by RA 233 treatment, whereas spontaneous metastasis from the mammary fat pad to lung sites was decreased, although this decrease was not statistically significant.
嘧啶并嘧啶类似物RA 233对从13762NF大鼠乳腺腺癌分离出的培养肿瘤细胞克隆具有多效性和差异性作用。低转移潜能的无反应克隆（MTC）经RA 233处理后，其细胞脆性、侵袭性、黏附性或肺定植特性均未改变。相比之下，高转移潜能的药物反应克隆（MTLn3）经RA 233处理后侵袭性降低，细胞脆性减小，尽管其对肺微血管内皮细胞和内皮下基质的黏附性不受RA 233影响。RA 233处理显著增加了同基因大鼠静脉注射MTLn3细胞后的肺定植，而从乳腺脂肪垫到肺部的自发转移减少，尽管这种减少没有统计学意义。
