Neri A, Welch D, Kawaguchi T, Nicolson G L
J Natl Cancer Inst. 1982 Mar;68(3):507-17.
With the use of the rat 13762 mammary adenocarcinoma tumor, an animal model for spontaneous mammary tumor metastasis was developed. The parental tumor implanted sc into the mammary fat pads of female Fischer 344 rats metastasized at low frequency to lymph nodes and lung and did not metastasize to other organs. Oophorectomy did not modify the metastatic properties of the parental 13762 tumor. Cell lines were adapted to tissue culture from lymph node or lung metastases, and these were compared to parental tumor transplants in spontaneous metastasis assays. Only the cell lines established from metastases were spontaneously metastatic within 23 days after sc implantation, indicating that tumor cell populations from metastases are more metastatic than are cells from the parental tumor. Several individual parental tumor-derived and lung metastasis-derived clones were compared for spontaneous metastatic potentials, cell culture morphologies, histologic structures at primary implant and secondary metastatic sites, and growth characteristics in vivo and in vitro. There was no correlation between any of these tumor and cellular properties and metastatic potential. The parental mammary tumor-derived, lymph node metastasis-derived, and lung metastasis-derived cell lines and clones with differing spontaneous metastatic properties should prove useful in studies on the roles of tumor cell and host properties in lymphatic and blood-borne metastasis.
利用大鼠13762乳腺腺癌肿瘤，建立了一种自发性乳腺肿瘤转移的动物模型。将亲代肿瘤皮下植入雌性Fischer 344大鼠的乳腺脂肪垫中，其转移至淋巴结和肺的频率较低，且不转移至其他器官。卵巢切除术并未改变亲代13762肿瘤的转移特性。从淋巴结或肺转移灶建立适应组织培养的细胞系，并在自发性转移试验中将其与亲代肿瘤移植进行比较。只有从转移灶建立的细胞系在皮下植入后23天内具有自发性转移能力，这表明来自转移灶的肿瘤细胞群体比亲代肿瘤细胞更具转移性。比较了几个亲代肿瘤来源和肺转移来源的克隆在自发性转移潜能、细胞培养形态、原发植入部位和继发转移部位的组织结构以及体内外生长特性等方面的差异。这些肿瘤和细胞特性与转移潜能之间均无相关性。具有不同自发性转移特性的亲代乳腺肿瘤来源、淋巴结转移来源和肺转移来源的细胞系及克隆，在研究肿瘤细胞和宿主特性在淋巴转移和血行转移中的作用方面应具有重要价值。
