Department of General and Specialized Surgery, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil.
Department of Physiology and Pharmacology, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil.
Int J Exp Pathol. 2017 Oct;98(5):269-277. doi: 10.1111/iep.12250. Epub 2017 Dec 4.
End-to-end anastomosis in the treatment for bile duct injury during laparoscopic cholecystectomy has been associated with stricture formation. The aim of this study was to experimentally investigate the effect of oral tamoxifen (tmx) treatment on fibrosis, collagen content and transforming growth factor-β1, -β2 and -β3 expression in common bile duct anastomosis of pigs. Twenty-six pigs were divided into three groups [sham (n = 8), control (n = 9) and tmx (n = 9)]. The common bile ducts were transected and anastomosed in the control and tmx groups. Tmx (40 mg/day) was administered orally to the tmx group, and the animals were euthanized after 60 days. Fibrosis was analysed by Masson's trichrome staining. Picrosirius red was used to quantify the total collagen content and collagen type I/III ratio. mRNA expression of transforming growth factor (TGF)-β1, -β2 and -β3 was quantified using real-time polymerase chain reaction (qRT-PCR). The control and study groups exhibited higher fibrosis than the sham group, and the study group showed lower fibrosis than the control group (P = 0.011). The control and tmx groups had higher total collagen content than the sham group (P = 0.003). The collagen type I/III ratio was higher in the control group than in the sham and tmx groups (P = 0.015). There were no significant differences in the mRNA expression of TGF-β1, -β2 and -β3 among the groups (P > 0.05). Tmx decreased fibrosis and prevented the change in collagen type I/III ratio caused by the procedure.
在腹腔镜胆囊切除术中，胆管损伤的端到端吻合与狭窄形成有关。本研究旨在实验性研究口服他莫昔芬（tmx）治疗对猪胆总管吻合口纤维化、胶原含量和转化生长因子-β1、-β2 和-β3 表达的影响。26 头猪分为三组[假手术组（n=8）、对照组（n=9）和 tmx 组（n=9）]。对照组和 tmx 组的胆总管被横断和吻合。tmx 组（40mg/天）口服给予 tmx，动物在 60 天后安乐死。通过 Masson 三色染色分析纤维化。苦味酸天狼星红用于定量总胶原含量和胶原 I/III 比值。采用实时聚合酶链反应（qRT-PCR）定量转化生长因子（TGF）-β1、-β2 和-β3 的 mRNA 表达。对照组和研究组的纤维化程度高于假手术组，研究组的纤维化程度低于对照组（P=0.011）。对照组和 tmx 组的总胶原含量高于假手术组（P=0.003）。对照组的胶原 I/III 比值高于假手术组和 tmx 组（P=0.015）。各组 TGF-β1、-β2 和-β3 的 mRNA 表达无显著差异（P>0.05）。tmx 可减少纤维化，并防止手术引起的胶原 I/III 比值变化。
