Fluminense Federal University, Department of General and Specialized Surgery, Niterói/RJ, Brazil.
Clinics (Sao Paulo). 2013 Jan;68(1):101-6. doi: 10.6061/clinics/2013(01)oa16.
The aim of this study was to evaluate the effect of oral tamoxifen treatment on the number of myofibroblasts present during the healing process after experimental bile duct injury.
The sample consisted of 16 pigs that were divided into two groups (the control and study groups). Incisions and suturing of the bile ducts were performed in the two groups. Tamoxifen (20 mg/day) was administered only to the study group. The animals were sacrificed after 30 days. Quantification of myofibroblasts in the biliary ducts was made through immunohistochemistry analysis using anti-alpha smooth muscle actin of the smooth muscle antibody. Immunohistochemical quantification was performed using a digital image system.
In the animals treated with tamoxifen (20 mg/day), there was a significant reduction in immunostaining for alpha smooth muscle actin compared with the control group (0.1155 vs. 0.2021, p = 0.046).
Tamoxifen reduced the expression of alpha smooth muscle actin in the healing tissue after bile duct injury, suggesting a decrease in myofibroblasts in the scarred area of the pig biliary tract. These data suggest that tamoxifen could be used in the prevention of biliary tract stenosis after bile duct surgeries.
本研究旨在评估口服他莫昔芬治疗对实验性胆管损伤后愈合过程中肌成纤维细胞数量的影响。
本样本由 16 头猪组成，分为两组（对照组和研究组）。两组均进行胆管切开和缝合。仅研究组给予他莫昔芬（20mg/天）治疗。30 天后处死动物。通过使用平滑肌抗α平滑肌肌动蛋白抗体的免疫组织化学分析对胆管中的肌成纤维细胞进行定量。使用数字图像系统进行免疫组织化学定量。
在接受他莫昔芬（20mg/天）治疗的动物中，与对照组相比，α平滑肌肌动蛋白免疫染色显著减少（0.1155 与 0.2021，p=0.046）。
他莫昔芬降低了胆管损伤后愈合组织中α平滑肌肌动蛋白的表达，提示猪胆管瘢痕区域的肌成纤维细胞减少。这些数据表明，他莫昔芬可用于预防胆管手术后的胆道狭窄。
