Department of Cardiology, Renmin Hospital of Wuhan University, Cardiovascular Research Institute, Wuhan University, Hubei Key Laboratory of Cardiology, Wuhan, Hubei 430060, P.R. China.
Int J Mol Med. 2018 Feb;41(2):845-851. doi: 10.3892/ijmm.2017.3259. Epub 2017 Nov 16.
Bone mesenchymal stem cells (BMSCs) are currently considered the optimal stem cells for biological pacemaker cell transformation. The cardiac‑specific transcription factor T‑Box protein 18 (TBX18) is essential for sinoatrial node (SAN) formation, particularly formation of the head region that generates the electrical impulses that induce heart contraction. The present study aimed to confirm the effects of TBX18 on biological pacemaker differentiation of rat BMSCs. Flow cytometry was used to identify the surface markers of BMSCs, in order to acquire pure mesenchymal stem cells. Subsequently, BMSCs were transduced with TBX18 or green fluorescent protein adenovirus vectors. The effects of TBX18 were evaluated using SAN‑specific makers including TBX18, α‑actin, cardiac troponin I, hyperpolarization‑activated cyclic nucleotide‑gated channel 4 and connexin 43 by reverse transcription‑quantitative polymerase chain reaction, western blotting and immunofluorescence. The findings demonstrated that direct conversion of BMSCs to biological pacemaker cells via TBX18 is a feasible method in the field of cardiology.
骨髓间充质干细胞（BMSCs）目前被认为是转化为生物起搏器细胞的最佳干细胞。心脏特异性转录因子 T 盒蛋白 18（TBX18）对于窦房结（SAN）的形成至关重要，特别是头部区域的形成，头部区域产生电脉冲，从而引发心脏收缩。本研究旨在证实 TBX18 对大鼠 BMSCs 生物起搏器分化的影响。采用流式细胞术鉴定 BMSCs 的表面标志物，以获得纯间充质干细胞。随后，用 TBX18 或绿色荧光蛋白腺病毒载体转导 BMSCs。通过逆转录-定量聚合酶链反应、Western blot 和免疫荧光，用 SAN 特异性标志物包括 TBX18、α-肌动蛋白、心肌肌钙蛋白 I、超极化激活环核苷酸门控通道 4 和连接蛋白 43 评估 TBX18 的作用。研究结果表明，通过 TBX18 将 BMSCs 直接转化为生物起搏器细胞是心脏病学领域的一种可行方法。
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