胸腺素单体性质的证据。

Evidence for the monomeric nature of thymosins.

作者信息

Haritos A A, Yialouris P P, Heimer E P, Felix A M, Hannappel E, Rosemeyer M A

机构信息

Zoological Laboratory, Faculty of Sciences, University of Athens, Greece.

出版信息

FEBS Lett. 1989 Feb 27;244(2):287-90. doi: 10.1016/0014-5793(89)80547-2.

DOI:10.1016/0014-5793(89)80547-2

PMID:2920828

Abstract

According to gel-filtration experiments, alpha- and beta-thymosins appear to form oligomers, which are 4-5-fold larger than the corresponding polypeptides. However, on analysis by sedimentation equilibrium ultracentrifugation, prothymosin alpha and thymosin beta 4 showed relative molecular masses of 12,800 and 4600, which are close to the values calculated from their amino acid sequences, confirming their existence in solution as discrete monomeric entities.

摘要

根据凝胶过滤实验，α-和β-胸腺素似乎形成了寡聚体，其大小比相应的多肽大4-5倍。然而，通过沉降平衡超速离心分析，前胸腺素α和胸腺素β4的相对分子质量分别为12,800和4600，这与根据它们的氨基酸序列计算的值接近，证实它们在溶液中以离散的单体形式存在。