编码含胸腺素α1序列的16000道尔顿多肽的胸腺信使核糖核酸的纯化
Purification of thymus mRNA coding for a 16,000-dalton polypeptide containing the thymosin alpha 1 sequence.
作者信息
Freire M, Hannappel E, Rey M, Freire J M, Kido H, Horecker B L
出版信息
Proc Natl Acad Sci U S A. 1981 Jan;78(1):192-5. doi: 10.1073/pnas.78.1.192.
Abstract
A mRNA fraction purified by preparative polyacrylamide disc gel electrophoresis from calf thymus polysomes codes for a polypeptide(s) having a mass of 16,000-17,000 daltons. This polypeptide contains amino acid sequences corresponding to residues 11-18 and 19-25 of thymosin alpha 1. The yield of the octapeptide indicates that the 16,000-dalton peptide is the major product formed in the cell-free synthesis system containing the purified mRNA.
摘要
通过制备性聚丙烯酰胺圆盘凝胶电泳从小牛胸腺多核糖体中纯化得到的一种mRNA组分编码一种分子量为16,000 - 17,000道尔顿的多肽。该多肽包含与胸腺素α1的第11 - 18位和19 - 25位残基相对应的氨基酸序列。八肽的产量表明，在含有纯化mRNA的无细胞合成系统中形成的主要产物是16,000道尔顿的肽。
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本文引用的文献
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