Nilsson Anders K, Andersson Mats X
Department of Biology and Environmental Sciences, University of Gothenburg, Gothenburg, Sweden.
PeerJ. 2017 Dec 1;5:e4134. doi: 10.7717/peerj.4134. eCollection 2017.
A striking and unexpected biochemical phenotype was found in an insertion mutant line in the model plant . One of two investigated insertion mutant lines in the gene encoding the phosphate transporter PHT4;1 demonstrated a prominent loss of trienoic fatty acids, whereas the other insertion line was indistinguishable from wild type in this aspect. We demonstrate that the loss of trienoic fatty acids was due to a remnant inactive negative selection marker gene in this particular transposon tagged line, . This constitutes a cautionary tale that warns of the importance to confirm the loss of this type of selection markers and the importance of verifying the relationship between a phenotype and genotype by more than one independent mutant line or alternatively genetic complementation.
在模式植物的一个插入突变体系中发现了一种显著且出乎意料的生化表型。在编码磷酸盐转运蛋白PHT4;1的基因中，两个被研究的插入突变体系之一表现出三烯脂肪酸的显著缺失，而另一个插入体系在这方面与野生型没有区别。我们证明，三烯脂肪酸的缺失是由于这个特定转座子标签系中残留的无活性阴性选择标记基因。这构成了一个警示故事，提醒人们确认此类选择标记缺失的重要性，以及通过多个独立突变体系或基因互补来验证表型与基因型之间关系的重要性。
