在动物细胞存在的情况下，由特内里费诺卡氏菌IFM 10554的共培养物分离得到的脱氢丙基泛酰胺。
Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554 in the presence of animal cells.
作者信息
Hara Yasumasa, Arai Midori A, Sakai Kanae, Ishikawa Naoki, Gonoi Tohru, Yaguchi Takashi, Ishibashi Masami
机构信息
Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8675, Japan.
Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8673, Japan.
出版信息
J Nat Med. 2018 Jan;72(1):280-289. doi: 10.1007/s11418-017-1161-y. Epub 2017 Dec 5.
A new amide, named dehydropropylpantothenamide (1), was obtained by a co-culture of Nocardia tenerifensis IFM 10554 in the presence of the mouse macrophage-like cell line J774.1 in modified Czapek-Dox (mCD) medium. Compound 1 was synthesized from D-pantothenic acid calcium salt in 6 steps. The absolute configuration of natural compound 1 was determined by comparisons of the optical rotation and CD spectra of synthetic 1. In the present study, a new method for producing secondary metabolites was demonstrated using a "co-culture" in which the genus Nocardia was cultured in the presence of an animal cell line.
一种名为脱氢丙基泛酰胺（1）的新型酰胺，是通过在改良的察氏培养基（mCD）中，在小鼠巨噬细胞样细胞系J774.1存在的情况下，对特内里费岛诺卡氏菌IFM 10554进行共培养而获得的。化合物1由D - 泛酸钙盐经6步合成。通过比较合成的1的旋光度和圆二色光谱，确定了天然化合物1的绝对构型。在本研究中，展示了一种利用“共培养”生产次生代谢产物的新方法，即在动物细胞系存在的情况下培养诺卡氏菌属。
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