Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2017 Dec 6;18(12):2639. doi: 10.3390/ijms18122639.
The pine wood nematode (PWN), , is the pathogen of pine wilt disease (PWD) and causes huge economic losses in pine forests and shows a remarkable ability to survive under unfavorable and changing environmental conditions. This ability may be related to autophagy, which is still poorly understood in . Our previous studies showed that autophagy exists in PWN. Therefore, we tested the effects of autophagy inducer rapamycin on PWN and the results revealed that the feeding rate and reproduction were significantly promoted on fungal mats. The gene expression patterns of and under the different stress were determined by quantitative reverse transcription PCR (qRT-PCR). We tested the effects of RNA interference on and in PWN during different periods of infection in inus . The results revealed that and may play roles in allowing PWN to adapt to changing environmental conditions and the virulence of PWN was influenced by the silence of autophagy-related genes and . These results provided fundamental information on the relationship between autophagy and PWN, and on better understanding of gene function of and in PWN.
松材线虫（PWN）是松材线虫病（PWD）的病原体，它给松林带来了巨大的经济损失，并表现出在不利和不断变化的环境条件下生存的显著能力。这种能力可能与自噬有关，而自噬在 中仍未被充分了解。我们之前的研究表明，自噬存在于 PWN 中。因此，我们测试了自噬诱导剂雷帕霉素对 PWN 的影响，结果表明在真菌垫上，摄食率和繁殖率显著提高。通过定量反转录 PCR（qRT-PCR）确定了 在不同应激下的基因表达模式。我们在 inus 中测试了 RNA 干扰对 PWN 不同感染期的 和 的影响。结果表明， 和 可能在允许 PWN 适应不断变化的环境条件方面发挥作用，而 PWN 的毒力受到自噬相关基因 和 沉默的影响。这些结果为自噬与 PWN 之间的关系以及更好地了解 PWN 中 和 的基因功能提供了基础信息。
