Sankolli G M, Joshi U M
Institute for Research in Reproduction, Bombay, India.
J Biol Stand. 1989 Jan;17(1):35-40. doi: 10.1016/0092-1157(89)90026-7.
The variation in the estimates of urinary pregnanediol glucuronide obtained by ELISA with 12 polyclonal antisera has been assessed. Of the 12 antisera, eight gave values comparable with each other and could be used interchangeably in the ELISA. Thus a large stock of polyclonal antibody which gave consistent values was generated and can now be used in diagnostic ELISA over a long period of time. This procedure is more economical than the development of monoclonal antibodies for the same purpose.
对采用12种多克隆抗血清通过酶联免疫吸附测定（ELISA）获得的尿孕二醇葡萄糖醛酸苷估计值的差异进行了评估。在这12种抗血清中，有8种给出的数值彼此相当，可在ELISA中互换使用。因此，产生了一大批给出一致数值的多克隆抗体，现在可在较长时间内用于诊断性ELISA。该方法比为同一目的开发单克隆抗体更经济。
