通过用[3H] - 和[14C]胸苷双标记及流式细胞术直接证明小鼠腺癌EO 771中的肿瘤细胞募集。
Tumor cell recruitment in the mouse adenocarcinoma EO 771 directly demonstrated by double labeling with [3H]- and [14C] thymidine and flow cytometry.
作者信息
Walter J, Maurer-Schultze B
机构信息
Institut für Medizinische Strahlenkunde, Universität Würzburg, Federal Republic of Germany.
出版信息
J Cancer Res Clin Oncol. 1989;115(1):53-60. doi: 10.1007/BF00391600.
Tumor cell recruitment in the mouse adenocarcinoma EO 771 after application of 1-beta-D-arabinofuranosylcytosine (AraCyt) has been directly demonstrated by double labeling with [3H]- and [14C]-thymidine. This method enables a quantitative study of the extent and time course of tumor cell recruitment in this solid mouse tumor. The results show that tumor cell recruitment is a continuous process that starts early after AraCyt application (about 4-8 h) and lasts about 1 day with a maximum at about 12 h after Ara-Cyt application. Up to 40% of all cells are recruited at that time. The recruited tumor cells re-enter the resting state after having passed through one cycle.
通过用[³H] - 和[¹⁴C] - 胸腺嘧啶核苷进行双重标记，已直接证明了在应用1-β-D-阿拉伯呋喃糖基胞嘧啶（阿糖胞苷）后小鼠腺癌EO 771中的肿瘤细胞募集情况。该方法能够对这种实体小鼠肿瘤中肿瘤细胞募集的程度和时间进程进行定量研究。结果表明，肿瘤细胞募集是一个持续的过程，在阿糖胞苷应用后早期（约4 - 8小时）开始，持续约1天，在阿糖胞苷应用后约12小时达到最大值。此时，高达40%的所有细胞被募集。募集的肿瘤细胞在经历一个周期后重新进入静止状态。
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