Lang W, Kienzle S, Georgii A
J Cancer Res Clin Oncol. 1983;105(1):55-61. doi: 10.1007/BF00391832.
An in vitro double-labeling technique for determination of kinetic growth parameters was tested; 13 renal sarcomas of rats were incubated after excision under standardized conditions with 14C- and 3H-thymidine successively. Another ten tumors were evaluated after in vivo double injection of 14C and 3H-thymidine into sarcoma-bearing animals. In both groups histoautoradiography was performed using a simple stripping-film method. Results including labeling indices, mitotic indices, length of DNA-synthesis phase and cell-production rate obtained by both methods were in excellent agreement. All values fitted those determined in earlier experiments using a continuous labeling and a fraction-labeled-mitoses method in vivo. Theoretical considerations on the calculation of kinetic parameters by double labeling have been added. The experimental results found in this study represent a base for evaluation of biopsies from human tumors.
对一种用于测定动力学生长参数的体外双标记技术进行了测试；13只大鼠肾肉瘤在切除后，于标准化条件下先后用14C-和3H-胸腺嘧啶核苷进行孵育。另外十只肿瘤在给荷肉瘤动物体内双注射14C和3H-胸腺嘧啶核苷后进行评估。两组均采用简单的剥脱膜法进行组织放射自显影。通过两种方法获得的包括标记指数、有丝分裂指数、DNA合成期长度和细胞产生率在内的结果高度一致。所有数值均与早期在体内使用连续标记和部分标记有丝分裂法所测定的值相符。还增加了关于通过双标记计算动力学参数的理论思考。本研究中发现的实验结果为评估人类肿瘤活检提供了依据。
