Cai Haiyuan, Zeng Yonghui
State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, China.
Aarhus Institute of Advanced Studies & Department of Environmental Science, Aarhus University, Aarhus, Denmark.
Stand Genomic Sci. 2017 Dec 2;12:69. doi: 10.1186/s40793-017-0284-9. eCollection 2017.
TH1-2 is a member of the family within isolated from cyanobacterial aggregates in a eutrophic lake. The draft genome comprises 3,711,627 bp and 3489 predicted protein-coding genes. The genome of strain TH1-2 has 270 genes encoding peptidases. And metallo and serine peptidases were found most frequently. A high number of genes encoding carbohydrate active enzymes (141 CAZymes) also present in strain TH1-2 genome. Among CAZymes, 47 glycoside hydrolase families, 37 glycosyl transferase families, 38 carbohydrate esterases families, nine auxiliary activities families, seven carbohydrate-binding modules families, and three polysaccharide lyases families were identified. Accordingly, strain TH1-2 has a high number of transporters (91), the dominated ones are ATP-binding cassette transporters (61) and TonB-dependent transporters (28). Major TBDTs are Group I, which consisted of transporters for various types of dissolved organic matter. These genome features indicate adaption to cyanobacterial aggregates microenvironments.
TH1-2是从富营养化湖泊中的蓝藻聚集体中分离出的该菌属的一个成员。基因组草图包含3,711,627个碱基对和3489个预测的蛋白质编码基因。TH1-2菌株的基因组有270个编码肽酶的基因。其中金属肽酶和丝氨酸肽酶最为常见。TH1-2菌株的基因组中还存在大量编码碳水化合物活性酶的基因（141个碳水化合物活性酶）。在碳水化合物活性酶中，鉴定出47个糖苷水解酶家族、37个糖基转移酶家族、38个碳水化合物酯酶家族、9个辅助活性家族、7个碳水化合物结合模块家族和3个多糖裂解酶家族。因此，TH1-2菌株有大量的转运蛋白（91个），其中主要是ATP结合盒转运蛋白（61个）和TonB依赖性转运蛋白（28个）。主要的TonB依赖性转运蛋白是第一组，由各种溶解有机物的转运蛋白组成。这些基因组特征表明其对蓝藻聚集体微环境的适应性。
