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从富营养化湖泊蓝藻聚集体中分离出的TH1-2的高质量基因组草图序列。

High-quality draft genome sequence of TH1-2 isolated from cyanobacterial aggregates in a eutrophic lake.

作者信息

Cai Haiyuan, Zeng Yonghui

机构信息

State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, China.

Aarhus Institute of Advanced Studies & Department of Environmental Science, Aarhus University, Aarhus, Denmark.

出版信息

Stand Genomic Sci. 2017 Dec 2;12:69. doi: 10.1186/s40793-017-0284-9. eCollection 2017.

Abstract

TH1-2 is a member of the family within isolated from cyanobacterial aggregates in a eutrophic lake. The draft genome comprises 3,711,627 bp and 3489 predicted protein-coding genes. The genome of strain TH1-2 has 270 genes encoding peptidases. And metallo and serine peptidases were found most frequently. A high number of genes encoding carbohydrate active enzymes (141 CAZymes) also present in strain TH1-2 genome. Among CAZymes, 47 glycoside hydrolase families, 37 glycosyl transferase families, 38 carbohydrate esterases families, nine auxiliary activities families, seven carbohydrate-binding modules families, and three polysaccharide lyases families were identified. Accordingly, strain TH1-2 has a high number of transporters (91), the dominated ones are ATP-binding cassette transporters (61) and TonB-dependent transporters (28). Major TBDTs are Group I, which consisted of transporters for various types of dissolved organic matter. These genome features indicate adaption to cyanobacterial aggregates microenvironments.

摘要

TH1-2是从富营养化湖泊中的蓝藻聚集体中分离出的该菌属的一个成员。基因组草图包含3,711,627个碱基对和3489个预测的蛋白质编码基因。TH1-2菌株的基因组有270个编码肽酶的基因。其中金属肽酶和丝氨酸肽酶最为常见。TH1-2菌株的基因组中还存在大量编码碳水化合物活性酶的基因(141个碳水化合物活性酶)。在碳水化合物活性酶中,鉴定出47个糖苷水解酶家族、37个糖基转移酶家族、38个碳水化合物酯酶家族、9个辅助活性家族、7个碳水化合物结合模块家族和3个多糖裂解酶家族。因此,TH1-2菌株有大量的转运蛋白(91个),其中主要是ATP结合盒转运蛋白(61个)和TonB依赖性转运蛋白(28个)。主要的TonB依赖性转运蛋白是第一组,由各种溶解有机物的转运蛋白组成。这些基因组特征表明其对蓝藻聚集体微环境的适应性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29d3/5712168/45190210cd06/40793_2017_284_Fig1_HTML.jpg

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