State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, China.
Key Laboratory of Microbial Engineering at the Institute of Biology, Henan Academy of Sciences, Zhengzhou, China.
Antonie Van Leeuwenhoek. 2017 Sep;110(9):1169-1177. doi: 10.1007/s10482-017-0889-4. Epub 2017 Jun 9.
A bacterial strain, TH1-2, was isolated from cyanobacterial aggregates in the eutrophic Lake Taihu, Jiangsu Province, China. Cells were observed to be Gram-negative, slightly curved and rod-shaped. Optimal growth was obtained at pH 7.0 (range 5.5-8.5) and 30 °C (range 20-37 °C) in trypticase soy broth (TSB) without NaCl. Growth was not observed in TSB with 1.0% (w/v) NaCl added. The cells were found to be positive for oxidase and catalase activities. The major fatty acids were identified as 3-hydroxy hexadecanoic acid (C 3-OH), C ω5c and summed feature 8 (consisting of C ω6c and cis-11-Octadecenoic acid (C ω7c). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The 16S rRNA gene sequence of strain TH1-2 was found to be phylogenetically related to those of Glycocaulis abyssi MCS 33 and Brevundimonas naejangsanensis BIO-TAS2-2 (90.5 and 90.0% similarity, respectively). The genomic G+C content of strain TH1-2 was 55.6 mol% based on whole genome calculations. Average nucleotide identities (ANI) and the digital DNA-DNA hybridizations (DDH) for complete genomes ranged from 66.84 to 67.32 and 21.3 to 31.8% between strain TH1-2 and type strains within the family Caulobacteraceae, higher than those between strain TH1-2 and the strains within the family Hyphomonadaceae. The phenotypic, chemotaxonomic and phylogenetic properties, and genome analysis, indicate that strain TH1-2 (=CGMCC 1.12979 = LMG 28362) represents a novel species in a new genus within the family Caulobacteraceae; thus, the name Aquidulcibacter paucihalophilus gen. nov., sp. nov., is proposed.
一株细菌菌株 TH1-2 从中国江苏省富营养化太湖的蓝藻聚集体中分离得到。细胞被观察为革兰氏阴性，略带弯曲的杆状。在无 NaCl 的胰蛋白酶大豆肉汤（TSB）中，最佳生长条件为 pH7.0（范围 5.5-8.5）和 30°C（范围 20-37°C）。在添加 1.0%（w/v）NaCl 的 TSB 中未观察到生长。细胞被发现氧化酶和过氧化氢酶活性呈阳性。主要脂肪酸被鉴定为 3-羟基十六烷酸（C3-OH）、Cω5c 和总和特征 8（由 Cω6c 和顺式-11-十八烯酸（Cω7c）组成）。主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油。菌株 TH1-2 的 16S rRNA 基因序列与 Glycocaulis abyssi MCS 33 和 Brevundimonas naejangsanensis BIO-TAS2-2 的序列最为相似（分别为 90.5%和 90.0%相似）。基于全基因组计算，菌株 TH1-2 的基因组 G+C 含量为 55.6mol%。菌株 TH1-2 与芽单胞菌科内的模式菌株之间的全基因组平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（DDH）值分别为 66.84-67.32%和 21.3-31.8%，高于菌株 TH1-2 与噬氢菌科内菌株之间的数值。表型、化学分类和系统发育特征以及基因组分析表明，菌株 TH1-2（=CGMCC 1.12979=LMG 28362）代表芽单胞菌科内一个新属的新种；因此，提出了一个新属的名称 Aquidulcibacter paucihalophilus gen. nov.，sp. nov.。
