Gan Siou Ting, Wong Wei Chee, Wong Choo Kien, Soh Aik Chin, Kilian Andrzej, Low Eng-Ti Leslie, Massawe Festo, Mayes Sean
Biotechnology Research Centre, School of Biosciences, The University of Nottingham Malaysia Campus, 43500, Semenyih, Selangor Darul Ehsan, Malaysia.
Biotechnology Research Centre, Advanced Agriecological Research Sdn. Bhd., 43500, Semenyih, Selangor Darul Ehsan, Malaysia.
J Appl Genet. 2018 Feb;59(1):23-34. doi: 10.1007/s13353-017-0420-7. Epub 2017 Dec 6.
Oil palm (Elaeis guineensis Jacq.) is an outbreeding perennial tree crop with long breeding cycles, typically 12 years. Molecular marker technologies can greatly improve the breeding efficiency of oil palm. This study reports the first use of the DArTseq platform to genotype two closely related self-pollinated oil palm populations, namely AA0768 and AA0769 with 48 and 58 progeny respectively. Genetic maps were constructed using the DArT and SNP markers generated in combination with anchor SSR markers. Both maps consisted of 16 major independent linkage groups (2n = 2× = 32) with 1399 and 1466 mapped markers for the AA0768 and AA0769 populations, respectively, including the morphological trait "shell-thickness" (Sh). The map lengths were 1873.7 and 1720.6 cM with an average marker density of 1.34 and 1.17 cM, respectively. The integrated map was 1803.1 cM long with 2066 mapped markers and average marker density of 0.87 cM. A total of 82% of the DArTseq marker sequence tags identified a single site in the published genome sequence, suggesting preferential targeting of gene-rich regions by DArTseq markers. Map integration of higher density focused around the Sh region identified closely linked markers to the Sh, with D.15322 marker 0.24 cM away from the morphological trait and 5071 bp from the transcriptional start of the published SHELL gene. Identification of the Sh marker demonstrates the robustness of using the DArTseq platform to generate high density genetic maps of oil palm with good genome coverage. Both genetic maps and integrated maps will be useful for quantitative trait loci analysis of important yield traits as well as potentially assisting the anchoring of genetic maps to genomic sequences.
油棕（Elaeis guineensis Jacq.）是一种异花授粉的多年生木本作物，育种周期长，通常为12年。分子标记技术可以极大地提高油棕的育种效率。本研究首次报道了使用DArTseq平台对两个亲缘关系密切的自花授粉油棕群体进行基因分型，即AA0768和AA0769，分别有48和58个后代。利用DArT和SNP标记结合锚定SSR标记构建了遗传图谱。两张图谱均由16个主要独立连锁群组成（2n = 2× = 32），AA0768群体和AA0769群体分别有1399和1466个定位标记，包括形态性状“壳厚度”（Sh）。图谱长度分别为1873.7和1720.6 cM，平均标记密度分别为1.34和1.17 cM。整合图谱长1803.1 cM，有2066个定位标记，平均标记密度为0.87 cM。总共82%的DArTseq标记序列标签在已发表的基因组序列中鉴定出一个位点，表明DArTseq标记优先靶向富含基因的区域。围绕Sh区域进行的更高密度图谱整合鉴定出了与Sh紧密连锁的标记，D.15322标记与形态性状相距0.24 cM，与已发表的SHELL基因转录起始位点相距5071 bp。Sh标记的鉴定证明了使用DArTseq平台生成具有良好基因组覆盖度的油棕高密度遗传图谱的稳健性。遗传图谱和整合图谱对于重要产量性状的数量性状位点分析以及潜在地辅助将遗传图谱锚定到基因组序列都将是有用的。
