用环捕捉 DNA-生物物理方法阐明 SMC 蛋白的机制。
Catching DNA with hoops-biophysical approaches to clarify the mechanism of SMC proteins.
机构信息
Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Delft, Netherlands.
出版信息
Nat Struct Mol Biol. 2017 Dec 7;24(12):1012-1020. doi: 10.1038/nsmb.3507.
Structural maintenance of chromosome (SMC) complexes are central regulators of chromosome architecture that are essential in all domains of life. For decades, the structural biology field has been debating how these conserved protein complexes use their intricate ring-like structures to structurally organize DNA. Here, we review the contributions of single-molecule biophysical approaches to resolving the molecular mechanism of SMC protein function.
结构维持染色体 (SMC) 复合物是染色体结构的核心调节剂，在所有生命领域都必不可少。几十年来，结构生物学领域一直在争论这些保守的蛋白质复合物如何利用其复杂的环状结构来对 DNA 进行结构组织。在这里，我们回顾了单分子生物物理方法在解决 SMC 蛋白功能的分子机制方面的贡献。
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