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低温条件下采用不同生物强化策略的地下污水渗透系统(SWIS)的氮去除性能及微生物群落变化。

Nitrogen removal performance and microbial community changes in subsurface wastewater infiltration systems (SWISs) at low temperature with different bioaugmentation strategies.

机构信息

College of Resources and Environmental Science, Hebei Agricultural University, Baoding 071000, PR China; Key Laboratory for Farmland Eco-Environment of Hebei Province, 071000 Baoding, PR China.

College of Resources and Environmental Science, Hebei Agricultural University, Baoding 071000, PR China; Key Laboratory for Farmland Eco-Environment of Hebei Province, 071000 Baoding, PR China.

出版信息

Bioresour Technol. 2018 Feb;250:603-610. doi: 10.1016/j.biortech.2017.11.089. Epub 2017 Dec 2.

DOI:10.1016/j.biortech.2017.11.089
PMID:29216573
Abstract

Poor nitrogen removal efficiency (mainly nitrate, NO-N) at low temperatures strongly limits application of subsurface wastewater infiltration systems (SWISs). Seven psychrophilic strains (heterotrophic nitrifying bacteria and aerobic denitrifying bacteria) were isolated and added to SWISs to investigate the effect of embedding and direct-dosing bioaugmentation strategies on sewage treatment performance at low temperature. Both bioaugmentation strategies improved ammonium (NH-N) removal efficiencies, and the embedding strategy also exhibited satisfactory NO-N and total nitrogen (TN) removal efficiencies. Pyrosequencing results of the bacterial 16S rRNA gene indicated that the embedding strategy significantly decreased the indigenous soil microbial diversity (p < .05) and altered the bacterial community structure, significantly increasing the relative abundance of Clostridia, which have good nitrate-reducing activity.

摘要

低温条件下脱氮效率低(主要是硝酸盐,NO-N)严重限制了地下污水渗透系统(SWISs)的应用。本研究从地下污水渗透系统中分离出 7 株嗜冷菌(异养硝化菌和好氧反硝化菌),并将其添加到地下污水渗透系统中,以研究包埋和直接投加生物强化策略对低温条件下污水的处理效果。两种生物强化策略均提高了氨氮(NH-N)的去除效率,包埋策略还表现出了令人满意的硝酸盐和总氮(TN)去除效率。细菌 16S rRNA 基因的焦磷酸测序结果表明,包埋策略显著降低了土著土壤微生物的多样性(p < .05),并改变了细菌群落结构,显著增加了具有良好硝酸盐还原活性的梭菌的相对丰度。

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