Thornton J G, Wells M, Hume W J
Department of Obstetrics and Gynaecology, University of Leeds, U.K.
J Pathol. 1989 Feb;157(2):109-15. doi: 10.1002/path.1711570205.
The duration of the S phase of the cell cycle (ts) in the glands and stroma of normal, hyperplastic, and malignant endometrium was measured in vitro using a double labelling technique. Endometrial organ cultures were incubated sequentially in tritiated thymidine (3HTdR) and bromodeoxyuridine (BrdU) each for 1 h. Labelled cells were visualized in tissue sections using 3HTdR autoradiography and immunohistochemistry with an anti-(BrdU) antibody. Three populations of cells, single labelled with either 3HTdR or BrdU, and double labelled cells, were easily identified and ts was calculated from both the rate of influx into, and efflux out of S. There were no significant differences in ts in the different tissue types or between influx and efflux in the glands. However, in the stroma ts calculated from the rate of efflux was significantly shorter than that calculated from the rate of influx, suggesting that during the cell culture period the rate of cell efflux from S was greater than influx. This is the opposite to what would be expected in an exponentially growing cell population and suggests that there may be an effect from the culturing conditions.
采用双标记技术在体外测量正常、增生性和恶性子宫内膜的腺体及基质中细胞周期S期的持续时间（ts）。将子宫内膜器官培养物依次置于氚标记胸腺嘧啶核苷（3HTdR）和溴脱氧尿苷（BrdU）中各孵育1小时。使用3HTdR放射自显影术和抗（BrdU）抗体免疫组化法在组织切片中观察标记细胞。易于识别三种细胞群体，即仅用3HTdR或BrdU单标记的细胞以及双标记细胞，并根据进入S期和流出S期的速率计算ts。不同组织类型的ts或腺体中流入和流出速率之间无显著差异。然而，在基质中，根据流出速率计算的ts显著短于根据流入速率计算的ts，这表明在细胞培养期间，从S期流出的细胞速率大于流入速率。这与指数生长细胞群体的预期情况相反，提示培养条件可能存在影响。
