Mehdi A, Bokhari S A, Yousuf N, Umerani A, Chughtai S, Hussain F, Raza A
Department of Internal Medicine, University of Cincinnati Medical Center, Ohio.
Anticancer Res. 1992 Sep-Oct;12(5):1443-6.
HL-60 cells were sequentially labeled with the thymidine analogues iododeoxyuridine (IUdR) and bromodeoxyuridine (BrdU). The labeling index (LI), the duration of S-phase (Ts) and the total cell cycle time (Tc) were measured immediately. It was therefore possible to predict the next time when the single versus double labeled cells would re-enter the S-phase. In our study, the Tc was calculated to be 20 hours. The third label, tritiated thymidine (3HTdR), was introduced at the predicted time of 20 hours to confirm the validity of the previously calculated Tc. The actual percentage of cells which were labeled by (3HTdR) was very similar to the predicted value. We conclude, therefore, that the calculated cell cycle time correlated well with the actual cell cycle time, at least in a controlled in vitro culture system. This novel triple label method validates our double-label technique developed for cell cycle measurements.
HL-60细胞先后用胸苷类似物碘脱氧尿苷（IUdR）和溴脱氧尿苷（BrdU）进行标记。立即测量标记指数（LI）、S期持续时间（Ts）和总细胞周期时间（Tc）。因此，可以预测单标记和双标记细胞下次重新进入S期的时间。在我们的研究中，计算得出Tc为20小时。在预测的20小时时间点引入第三个标记，即氚标记的胸腺嘧啶核苷（3HTdR），以确认先前计算的Tc的有效性。被（3HTdR）标记的细胞的实际百分比与预测值非常相似。因此，我们得出结论，计算出的细胞周期时间与实际细胞周期时间相关性良好，至少在受控的体外培养系统中是这样。这种新颖的三重标记方法验证了我们为细胞周期测量开发的双标记技术。
