印度蜂胶的标准化、抗癌潜力及生物安全性
Standardization, anti-carcinogenic potential and biosafety of Indian propolis.
作者信息
Kapare Harshad, Lohidasan Sathiyanarayanan, Sinnathambi Arulmozhi, Mahadik Kakasaheb
机构信息
Department of Quality Assurance Techniques, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune 411038, India.
Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune 411038, India.
出版信息
J Ayurveda Integr Med. 2019 Apr-Jun;10(2):81-87. doi: 10.1016/j.jaim.2017.06.003. Epub 2017 Dec 6.
BACKGROUND
Propolis from apiculture is known for wide range of medicinal properties owing to its vast chemical constituents including polyphenols, flavonoids and anticancer agent Caffeic acid phenethyl ester (CAPE).
OBJECTIVES
The objective of the study was to extract and standardize Indian propolis (IP) with respect to selected markers by newly developed High performance liquid chromatography (HPLC) method, to evaluate in vitro and in vivo anticancer activity and biosafety of Indian propolis.
MATERIALS AND METHODS
IP was extracted, optimized and standardized using a newly developed and validated HPLC method for simultaneous estimation of caffeic acid, apigenin, quercetin and CAPE. The standardised ethanolic extract of IP (EEIP) was screened for in vitro cytotoxicity using sulforhodamine B (SRB) assay, in vivo anti-carcinogenic effect against Dalton's Lymphoma ascites (DLA) cells, hemolytic effect and pesticide analysis.
RESULTS
The EEIP was found to contain more amount of total flavonoids (23.61 ± 0.0452 mg equivalent of quercetin/g), total polyphenolics (34.82 ± 0.0785 mg equivalent of gallic acid/g) and all selected markers except caffeic acid compared to all other extracts. EEIP showed better anti-cancer potential than CAPE on MCF-7 and HT-29 cell line and significant (p < 0.01) in vivo anti-carcinogenic effects against DLA in comparison with 5-fluorouracil. EEIP was found to be non-hemolytic.
CONCLUSION
From in vitro cytotoxicity, in vivo anti-carcinogenicity and biosafety studies it can be concluded that the standardized EEIP is safe and can be considered for further development as a biomedicine.
背景
养蜂业中的蜂胶因其含有包括多酚、黄酮类化合物和抗癌剂咖啡酸苯乙酯（CAPE）在内的大量化学成分而具有广泛的药用特性。
目的
本研究的目的是通过新开发的高效液相色谱（HPLC）方法，针对选定的标志物对印度蜂胶（IP）进行提取和标准化，评估印度蜂胶的体外和体内抗癌活性及生物安全性。
材料与方法
采用新开发并经验证的HPLC方法对IP进行提取、优化和标准化，以同时测定咖啡酸、芹菜素、槲皮素和CAPE。使用磺酰罗丹明B（SRB）测定法对标准化的IP乙醇提取物（EEIP）进行体外细胞毒性筛选，评估其对道尔顿淋巴瘤腹水（DLA）细胞的体内抗癌作用、溶血作用和农药分析。
结果
与所有其他提取物相比，发现EEIP含有更多的总黄酮（23.61±0.0452毫克槲皮素当量/克）、总多酚（34.82±0.0785毫克没食子酸当量/克），且除咖啡酸外的所有选定标志物含量也更高。与CAPE相比，EEIP在MCF-7和HT-29细胞系上显示出更好的抗癌潜力，并且与5-氟尿嘧啶相比，对DLA具有显著（p<0.01）的体内抗癌作用。发现EEIP无溶血作用。
结论
从体外细胞毒性、体内抗癌性和生物安全性研究可以得出结论，标准化的EEIP是安全的，可考虑进一步开发为生物药物。
Zotero 插件