Kapare Harshad S, Giram Prabhanjan S, Raut Sadhana S, Gaikwad Hemant K, Paiva-Santos Ana Cláudia
Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune 411018, India.
Department of Pharmaceutical Sciences, The State University of New York, Buffalo, NY 14214, USA.
Gels. 2023 May 1;9(5):375. doi: 10.3390/gels9050375.
Flavonoids and polyphenolic compounds play a key role in wound healing cycle modulation. Propolis, a natural bee product, has been widely reported as an enriched source of polyphenols and flavonoids as important chemical constituents and for its wound healing potential. The goal of this study was to develop and characterize a propolis-based polyvinyl alcohol (PVA) hydrogel composition with wound healing potential. To understand the impacts of critical material attributes and process parameters, formulation development was carried out using a design of experiment approach. A preliminary phytochemical analysis of Indian propolis extract showed the presence of flavonoids (23.61 ± 0.0452 mg equivalent of quercetin/g) and polyphenols (34.82 ± 0.0785 mg equivalent of gallic acid/g), both of which aid in wound healing and skin tissue regeneration. The pH, viscosity, and in vitro release of the hydrogel formulation were also studied. The burn wound healing model results revealed significant ( < 0.0001) wound contraction by propolis hydrogel (93.58 + 0.15%) with rapid re-epithelialization relative to 5% / povidone iodine ointment USP (Cipladine) (95.39 + 0.16%). The excision wound healing model confirms significant ( < 0.0001) wound contraction by propolis hydrogel (91.45 + 0.29%) with accelerated re-epithelialization comparable to 5% / povidone iodine ointment USP (Cipladine) (94.38 + 0.21%). The developed formulation offers promise for wound healing, which may be investigated further for clinical research.
黄酮类化合物和多酚类化合物在伤口愈合周期调节中起关键作用。蜂胶是一种天然蜂产品，作为富含多酚和黄酮类化合物的重要化学成分来源及其伤口愈合潜力，已被广泛报道。本研究的目的是开发并表征一种具有伤口愈合潜力的基于蜂胶的聚乙烯醇（PVA）水凝胶组合物。为了解关键材料属性和工艺参数的影响，采用实验设计方法进行配方开发。对印度蜂胶提取物的初步植物化学分析表明存在黄酮类化合物（23.61±0.0452毫克槲皮素当量/克）和多酚类化合物（34.82±0.0785毫克没食子酸当量/克），两者均有助于伤口愈合和皮肤组织再生。还研究了水凝胶配方的pH值、粘度和体外释放情况。烧伤伤口愈合模型结果显示，与5%聚维酮碘软膏USP（西普拉定）（95.39±0.16%）相比，蜂胶水凝胶（93.58±0.15%）能显著（<0.0001）促进伤口收缩且上皮再形成迅速。切除伤口愈合模型证实，与5%聚维酮碘软膏USP（西普拉定）（94.38±0.21%）相比，蜂胶水凝胶（91.45±0.29%）能显著（<0.0001）促进伤口收缩且上皮再形成加速。所开发的配方为伤口愈合带来了希望，可进一步进行临床研究。
