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通过DNA相互作用荧光探针研究水溶液和人胚肾细胞（HEK）细胞核中甲基汞（MeHg）和汞（Hg）的化学形态。

Chemical speciation of MeHg and Hg in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes.

作者信息

Díaz de Greñu Borja, García-Calvo José, Cuevas José, García-Herbosa Gabriel, García Begoña, Busto Natalia, Ibeas Saturnino, Torroba Tomás, Torroba Blanca, Herrera Antonio, Pons Sebastian

机构信息

Department of Chemistry , Faculty of Science , University of Burgos , 09001 Burgos , Spain . Email:

Molecular Biology Institute of Barcelona , IBMB-CSIC , Barcelona Science Park , 08028 Barcelona , Spain.

出版信息

Chem Sci. 2015 Jul 1;6(7):3757-3764. doi: 10.1039/c5sc00718f. Epub 2015 Apr 30.

DOI:10.1039/c5sc00718f
PMID:29218145
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5707467/
Abstract

Selected new fluorogenic probes that interact in different ways with Hg and MeHg have been prepared and used for the chemical speciation of both cations in aqueous solution as well as in HEK293 cells. The best selective speciation of Hg and MeHg has been achieved by approaches based on fluorogenic probes supported in cultured cells, due to the particular sensitivity of the HEK293 cells to permeation by Hg, MeHg and the fluorogenic probes. In particular, MeHg was selectively detected in cell nuclei by probe JG45.

摘要

已制备出多种以不同方式与汞（Hg）和甲基汞（MeHg）相互作用的新型荧光探针，并将其用于水溶液以及人胚肾293（HEK293）细胞中这两种阳离子的化学形态分析。由于HEK293细胞对Hg、MeHg和荧光探针的渗透具有特殊敏感性，基于培养细胞中支持的荧光探针的方法实现了对Hg和MeHg的最佳选择性形态分析。特别是，探针JG45在细胞核中选择性地检测到了MeHg。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/edb0618bcd01/c5sc00718f-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/41e864004410/c5sc00718f-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/179ac6518242/c5sc00718f-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/f09e9a8caa05/c5sc00718f-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/7c79f4b8f857/c5sc00718f-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/b460a40a8f4b/c5sc00718f-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/501fd572b98d/c5sc00718f-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/64184e8eca99/c5sc00718f-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/a5fd76e0ff32/c5sc00718f-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/edb0618bcd01/c5sc00718f-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/41e864004410/c5sc00718f-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/179ac6518242/c5sc00718f-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/f09e9a8caa05/c5sc00718f-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/7c79f4b8f857/c5sc00718f-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/b460a40a8f4b/c5sc00718f-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/501fd572b98d/c5sc00718f-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/64184e8eca99/c5sc00718f-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/a5fd76e0ff32/c5sc00718f-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d35/5707467/edb0618bcd01/c5sc00718f-f7.jpg

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