Welkenhuysen Niek, Adiels Caroline B, Goksör Mattias, Hohmann Stefan
Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
Department of Physics, University of Gothenburg, Gothenburg, Sweden.
Methods Mol Biol. 2018;1713:109-121. doi: 10.1007/978-1-4939-7507-5_9.
Microfluidic systems in combination with microscopy (e.g., fluorescence) can be a powerful tool to study, at single-cell level, the behavior and morphology of biological cells after uptake of glucose. Here, we briefly discuss the advantages of using microfluidic systems. We further describe how microfluidic systems are fabricated and how they are utilized. Finally, we discuss how the large amount of data can be analyzed in a "semi-automatic" manner using custom-made software. In summary, we provide a guide to how to use microfluidic systems in single-cell studies.
微流控系统与显微镜（如荧光显微镜）相结合，可以成为在单细胞水平上研究生物细胞摄取葡萄糖后的行为和形态的强大工具。在此，我们简要讨论使用微流控系统的优势。我们进一步描述微流控系统是如何制造的以及如何被利用。最后，我们讨论如何使用定制软件以“半自动”方式分析大量数据。总之，我们提供了一份关于如何在单细胞研究中使用微流控系统的指南。
