Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
Appl Biochem Biotechnol. 2018 Jun;185(2):541-554. doi: 10.1007/s12010-017-2668-0. Epub 2017 Dec 8.
Exopolysaccharide of Lachnum YM130 (LEP) was purified by diethylaminoethyl cellulose 52 and Sepharose CL-6B column chromatography. LEP-2a was identified to be a homogeneous component with an average molecular weight of 1.31 × 10 Da, which was consisted of mannose and galactose in a molar ratio of 3.8:1.0. The structure of LEP-2a was characterized by methylation analysis, FT-IR analysis, and NMR analysis. Results indicated that LEP-2a was a galactomannan with a backbone, composed of 1,2-linked-α-D-Manp, 1,2,6-linked-α-D-Manp, 1,3,4-linked-α-D-Manp, and 1,3-linked-β-D-Galp, which was substituted at O-2, O-3, O-4, and O-6 by branches. In vitro antitumor activity assay proved that LEP-2a could significantly enhance the inhibitory effectiveness of 5-FU on Hela cells at the concentrations of 100, 200, 300, and 400 μg/mL. The above results suggested that LEP-2a could be seen as a potential source for developing novel antineoplastic agents.
莱姆木 YM130 的胞外多糖（LEP）通过二乙基氨基乙基纤维素 52 和琼脂糖 CL-6B 柱色谱法进行纯化。LEP-2a 被鉴定为一种均相组分，平均分子量为 1.31×10 Da，由甘露糖和半乳糖以 3.8:1.0 的摩尔比组成。LEP-2a 的结构通过甲基化分析、FT-IR 分析和 NMR 分析进行了表征。结果表明，LEP-2a 是一种半乳甘露聚糖，具有由 1,2-连接的-α-D-Manp、1,2,6-连接的-α-D-Manp、1,3,4-连接的-α-D-Manp 和 1,3-连接的-β-D-Galp 组成的主链，这些糖通过支链在 O-2、O-3、O-4 和 O-6 处取代。体外抗肿瘤活性测定证明，LEP-2a 在 100、200、300 和 400 μg/mL 浓度下可显著增强 5-FU 对 Hela 细胞的抑制作用。上述结果表明，LEP-2a 可以作为开发新型抗肿瘤药物的潜在来源。
