Li Hongyan, Yu He, Zhu Huajie
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding, China.
College of Chemistry and Environment Science, Hebei University, Baoding, China.
Appl Biochem Biotechnol. 2017 May;182(1):128-141. doi: 10.1007/s12010-016-2315-1. Epub 2016 Nov 16.
An extracellular polysaccharide TP1A was purified from the fermented broth of Trichoderma sp. KK19L1 by combination of Q Sepharose fast flow and Sephacryl S-300 chromatography. TP1A was composed of Man, Gal, and Glc in a molar ratio of about 3.0:5.1:8.1. The molar mass of TP1A was about 40.0 kDa. Methylation and NMR analysis indicated that the probable structure of TP1A was [→4,6)-α-D-Glcp(1→6)-β-D-Galf(1→6)-β-D-Galf(1→2,6)-β-D-Galf(1→2,6)-β-D-Galf(1→2,6)-β-D-Galf(1→2,6)-α-D-Manp(1→2,6)-α-D-Manp(1→] with [α-D-Glcp(1→] and [α-D-Manp(1→6)-α-D-Glcp(1→6)-α-D-Glcp(1→] as branches. The antitumor study showed that TP1A was able to inhibit the cell viability of HeLa and MCF-7 cells. TP1A could arrest HeLa cells in G2/M phase and induce HeLa cell apoptosis. These findings suggest that fungal polysaccharides could be a potential source for antitumor agents.
通过Q Sepharose快速流动柱和Sephacryl S - 300色谱相结合的方法，从木霉菌株KK19L1的发酵液中纯化得到一种细胞外多糖TP1A。TP1A由甘露糖（Man）、半乳糖（Gal）和葡萄糖（Glc）组成，摩尔比约为3.0:5.1:8.1。TP1A的摩尔质量约为40.0 kDa。甲基化和核磁共振分析表明，TP1A的可能结构为[→4,6)-α-D-葡萄糖吡喃糖基(1→6)-β-D-半乳糖吡喃糖基(1→6)-β-D-半乳糖吡喃糖基(1→2,6)-β-D-半乳糖吡喃糖基(1→2,6)-β-D-半乳糖吡喃糖基(1→2,6)-β-D-半乳糖吡喃糖基(1→2,6)-α-D-甘露糖吡喃糖基(1→2,6)-α-D-甘露糖吡喃糖基(1→]，以[α-D-葡萄糖吡喃糖基(1→]和[α-D-甘露糖吡喃糖基(1→6)-α-D-葡萄糖吡喃糖基(1→6)-α-D-葡萄糖吡喃糖基(1→]作为分支。抗肿瘤研究表明，TP1A能够抑制HeLa和MCF - 7细胞的细胞活力。TP1A可使HeLa细胞停滞于G2/M期并诱导HeLa细胞凋亡。这些发现表明真菌多糖可能是抗肿瘤药物的潜在来源。
