Institute of Bioscience, Universiti Putra, Serdang, Selangor, Malaysia.
Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra, Serdang, Selangor, Malaysia.
Rev Neurosci. 2018 Mar 28;29(3):321-332. doi: 10.1515/revneuro-2017-0069.
Injury to tissues is a major clinical challenge due to the limited regenerative capacity of endogenous cells. Stem cell therapy is evolving rapidly as an alternative for tissue regeneration. However, increasing evidence suggests that the regenerative ability of stem cells is mainly mediated by paracrine actions of secretome that are generally secreted by the cells. We aimed to systematically evaluate the efficacy of dental stem cell (DSC)-conditioned medium in in vivo animal models of various tissue defects. A total of 15 eligible studies was included by searching Pubmed, Scopus and Medline databases up to August 2017. The risk of bias was assessed using the Systematic Review Centre for Laboratory Animal Experimentation risk of bias tool. Of 15 studies, seven reported the therapeutic benefit of the conditioned medium on neurological diseases and three reported on joint/bone-related defects. Two interventions were on liver diseases, whereas the remaining three addressed myocardial infarction and reperfusion, lung injury and diabetes. Nine studies were performed using mouse models and the remaining six studies used rat models. The methodological quality of the studies was low, as most of the key elements required in reports of preclinical studies were not reported. The findings of this review suggested that conditioned medium from DSCs improved tissue regeneration and functional recovery. This current review strengthens the therapeutic benefit of cell-free product for tissue repair in animal models. A well-planned study utilizing validated outcome measures and long-term safety studies are required for possible translation to clinical trials.
由于内源性细胞的再生能力有限，组织损伤是一个主要的临床挑战。干细胞疗法作为组织再生的替代方法正在迅速发展。然而，越来越多的证据表明，干细胞的再生能力主要是通过细胞分泌的细胞外基质的旁分泌作用来介导的。我们旨在系统地评估牙源性干细胞（DSC）条件培养基在各种组织缺损的体内动物模型中的疗效。通过搜索 Pubmed、Scopus 和 Medline 数据库，截至 2017 年 8 月，共纳入了 15 项符合条件的研究。使用系统性综述中心实验室动物实验偏倚风险工具评估了偏倚风险。在 15 项研究中，有 7 项报告了条件培养基对神经疾病的治疗益处，有 3 项报告了关节/骨骼相关缺陷。有 2 项干预措施针对肝脏疾病，而其余 3 项则针对心肌梗死和再灌注、肺损伤和糖尿病。9 项研究使用了小鼠模型，其余 6 项研究使用了大鼠模型。研究的方法学质量较低，因为大多数临床前研究报告中需要的关键要素都没有报告。本综述的结果表明，DSC 的条件培养基改善了组织再生和功能恢复。这一当前综述加强了无细胞产品在动物模型中用于组织修复的治疗益处。需要进行精心设计的研究，使用经过验证的结局指标和长期安全性研究，以便可能转化为临床试验。
