College of Bioscience and Biotechnology, Jiangxi Agricultural University, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang 330045, PR China; Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources, Jiangxi Agricultural University, Nanchang 330045, PR China.
College of Bioscience and Biotechnology, Jiangxi Agricultural University, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang 330045, PR China; Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources, Jiangxi Agricultural University, Nanchang 330045, PR China.
Bioresour Technol. 2018 Feb;250:650-657. doi: 10.1016/j.biortech.2017.11.092. Epub 2017 Nov 29.
The biodegradation and detoxification of azo dye - Direct Black G (DBG) with a newly isolated thermophilic microflora was investigated in the present study. It was found this microflora can decolorize DBG at a wide range of pH from 5 to 10, and grow well under high concentration of dye (600 mg·L) and salinity (50 g·L). Its decolorization ratio could reach 97% with 8 h of incubation at optimal conditions. The induction of laccase, manganese peroxidase, lignin peroxidase and azoreductase suggests their synergetic involvements in the degradation process of DBG. In addition, the phytotoxicity analysis indicated the thermophilic microflora converted toxic dye DBG into low toxicity metabolites. PCR-DGGE analysis revealed that there are nine different bacteria presented in this microflora. Furthermore, a new degradation pathway of DBG degradation by this microflora was proposed based on the intermediates identified by LC-ESI-MS/MS.
本研究考察了一种新分离的嗜热微生物菌群对偶氮染料直接黑 G（DBG）的生物降解和解毒作用。研究发现，该微生物菌群在 pH 值为 5 至 10 的较宽范围内可使 DBG 脱色，并且在高浓度染料（600mg·L）和盐度（50g·L）条件下生长良好。在最佳条件下孵育 8 小时，其脱色率可达 97%。漆酶、锰过氧化物酶、木质素过氧化物酶和偶氮还原酶的诱导表明它们在 DBG 降解过程中协同作用。此外，植物毒性分析表明，嗜热微生物菌群将毒性染料 DBG 转化为低毒性代谢物。PCR-DGGE 分析表明，该微生物菌群中存在 9 种不同的细菌。此外，根据 LC-ESI-MS/MS 鉴定的中间产物，提出了该微生物菌群降解 DBG 的新途径。
