Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400 UPM, Malaysia.
Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi 43600 UKM, Malaysia.
Int J Environ Res Public Health. 2021 Mar 2;18(5):2424. doi: 10.3390/ijerph18052424.
The application of microorganisms in azo dye remediation has gained significant attention, leading to various published studies reporting different methods for obtaining the best dye decolouriser. This paper investigates and compares the role of methods and media used in obtaining a bacterial consortium capable of decolourising azo dye as the sole carbon source, which is extremely rare to find. It was demonstrated that a prolonged acclimation under low substrate availability successfully isolated a novel consortium capable of utilising Reactive Red 120 dye as a sole carbon source in aerobic conditions. This consortium, known as JR3, consists of strain MM01, strain MM05 and strain MM06. Decolourised metabolites of consortium JR3 showed an improvement in mung bean's seed germination and shoot and root length. One-factor-at-time optimisation characterisation showed maximal of 82.9% decolourisation at 0.7 g/L ammonium sulphate, pH 8, 35 °C, and RR120 concentrations of 200 ppm. Decolourisation modelling utilising response surface methodology (RSM) successfully improved decolourisation even more. RSM resulted in maximal decolourisation of 92.79% using 0.645 g/L ammonium sulphate, pH 8.29, 34.5 °C and 200 ppm RR120.
微生物在偶氮染料修复中的应用引起了广泛关注，导致了各种已发表的研究报告不同的方法，以获得最佳的染料脱色剂。本文研究并比较了获得能够以偶氮染料为唯一碳源脱色的细菌群落的方法和介质的作用，这是极其罕见的。研究表明，在低基质可用性条件下进行长时间驯化，成功分离出一种能够在好氧条件下利用活性红 120 染料作为唯一碳源的新型群落。该群落被称为 JR3，由菌株 MM01、菌株 MM05 和菌株 MM06 组成。群落 JR3 的脱色代谢产物显示出对绿豆种子发芽和幼苗及根长的改善。单因素优化特性表明，在 0.7 g/L 硫酸铵、pH 8、35°C 和 200 ppm RR120 浓度下，最大脱色率为 82.9%。利用响应面法（RSM）的脱色建模甚至成功地提高了脱色率。RSM 使最大脱色率达到 92.79%，使用 0.645 g/L 硫酸铵、pH 8.29、34.5°C 和 200 ppm RR120。
