Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh.
Laboratory of Organic Chemistry and Chemical Biology, Department of Chemistry, University of Turku, Turku FI-20014, Finland.
J Ethnopharmacol. 2018 Mar 25;214:8-12. doi: 10.1016/j.jep.2017.11.038. Epub 2017 Dec 5.
Bambusa arundinacea (Retz.) Willd., commonly known as Kanta Bans, plays an important ethnobotanical role, especially in Asia. In traditional medicine it has reportedly been used for the treatment of constipation, blood-diseases, leucodema, inflammation and urinary discharges. A number of ethnomedicinal records exist regarding the use of B. arundinaceae shoots as a laxative, however, there are no scientific studies reported on its laxative activity. Therefore, the aim of this present study was to evaluate the laxative activity of an ethanolic B. arundinaceae shoot extract in mice.
B. arundinacea shoots were collected from Dhaka, Bangladesh in July 2015. An ethanolic shoot extract was obtained and its laxative activity was evaluated by faecal consistency, gastrointestinal transit and entero-pooling assays in a mouse model. Furthermore, a phytochemical investigation of the extract was conducted by UHPLC-ESI-QqQ MS and UHPLC-ESI-Orbitrap MS analysis.
The ethanolic shoot extract of B. arundinacea showed significant laxative activity in our mouse model, with significant increases in (i) the amount of wet faeces, with the maximum effect at 2h for 500mg/kg (47.92%), (ii) gastrointestinal transit (67.18% and 60.03% for doses of 250 and 500mg/kg, respectively), and (iii) small intestine content at the test doses of 250 and 500mg/kg p.o. Phytochemical investigation identified a total of thirty compounds in the ethanolic shoot extract of B. arundinacea using UHPLC-ESI-QqQ MS and UHPLC-ESI-Orbitrap MS analysis.
The results of this study provide support for the traditional use of B. arundinacea shoot as a laxative.
Bambusa arundinacea (Retz.) Willd.，俗称 Kanta Bans，在亚洲具有重要的民族植物学作用。在传统医学中，据报道，它被用于治疗便秘、血液疾病、白癜、炎症和尿漏。有许多关于 B. arundinaceae 嫩枝用作泻药的民族医学记录，然而，没有关于其通便活性的科学研究报告。因此，本研究旨在评估 B. arundinaceae 嫩枝乙醇提取物在小鼠中的通便活性。
B. arundinacea 嫩枝于 2015 年 7 月从孟加拉国达卡采集。获得乙醇嫩枝提取物，并通过粪便一致性、胃肠道转运和肠池形成试验在小鼠模型中评估其通便活性。此外，通过 UHPLC-ESI-QqQ MS 和 UHPLC-ESI-Orbitrap MS 分析对提取物进行了植物化学研究。
B. arundinacea 的乙醇嫩枝提取物在我们的小鼠模型中表现出显著的通便活性，显著增加了（i）湿粪便的数量，最大作用在 2h 时为 500mg/kg（47.92%），（ii）胃肠道转运（剂量为 250 和 500mg/kg 时分别为 67.18%和 60.03%），以及（iii）口服 250 和 500mg/kg 时的小肠内容物。使用 UHPLC-ESI-QqQ MS 和 UHPLC-ESI-Orbitrap MS 分析，在 B. arundinacea 的乙醇嫩枝提取物中总共鉴定出 30 种化合物。
本研究结果为 B. arundinacea 嫩枝作为泻药的传统用途提供了支持。
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