Mangunwidjaja Djumali, Raharja Sapta, Kardono Leonardus B S, Iswantini Dyah
Department of Agroindustrial Technology, Faculty of Agricultural Technology, Bogor Agricultural University, Bogor, 16002, Indonesia.
Pak J Biol Sci. 2008 Feb 15;11(4):618-22. doi: 10.3923/pjbs.2008.618.622.
Identification and taxonomy analysis conducted at Herbarium Bogoriense at Research Centre for Biology, Indonesian Institute of Sciences Bogor. The name of the plant was C. tiglium L. The result of analysis on C. tiglium, ethanol extract as laxative material using the intestinal transit method showed treatment group that received dosage 0.06 mL/30 g b.wt. (72.5%) was significantly different compared to negative control (48.4%) or positive control (50.6%) which showed the weak effect as laxative at the dosage of 0.75 mL/30 g b.wt. It showed that ethanol extract of C. tiglium seed at dosage 0.06 mL/30 g is effective as laxative. The test result of the treatment using dosage 0.06, 0.04, 0.026 and 0.07 mL/28 g of body weight showed the mice population response 100, 60, 40 and 40% consecutively. The Thompson and Weil analysis result showed the ED50 was at 0.027 mL or equal to 639,5 g kg(-1) b.wt. The LD50 was at 0.0707 equals with 1674,5 mg kg(-1) b.wt. Safety limit is the range of dosage that cause the lethal effect and the dosage that gives the intended effect. The safety limit is represented by the comparison of LD50/ED50. Calculation result that the extract safety limit was LD50/ED50 = 0.0707/0.027 = 2.7.
在位于茂物的印度尼西亚科学院生物学研究中心的茂物植物标本馆进行了鉴定和分类分析。该植物名为巴豆（C. tiglium L.）。使用肠道运输法对巴豆乙醇提取物作为泻药材料的分析结果表明，接受剂量为0.06 mL/30 g体重的治疗组（72.5%）与阴性对照组（48.4%）或阳性对照组（50.6%）相比有显著差异，阳性对照组在剂量为0.75 mL/30 g体重时显示出较弱的泻药效果。这表明剂量为0.06 mL/30 g的巴豆种子乙醇提取物作为泻药是有效的。使用剂量为0.06、0.04、0.026和0.07 mL/28 g体重进行治疗的测试结果显示，小鼠群体反应依次为100%、60%、40%和40%。汤普森和韦尔分析结果表明，半数有效剂量（ED50）为0.027 mL或相当于639.5 g kg⁻¹体重。半数致死剂量（LD50）为0.0707，相当于1674.5 mg kg⁻¹体重。安全限度是指引起致死效应的剂量范围和产生预期效果的剂量范围。安全限度由LD50/ED50的比值表示。计算结果表明提取物的安全限度为LD50/ED50 = 0.0707/0.027 = 2.7。
