Bianchi M E, Beltrame M, Paonessa G
European Molecular Biology Laboratory, Heidleberg, Federal Republic of Germany.
Science. 1989 Feb 24;243(4894 Pt 1):1056-9. doi: 10.1126/science.2922595.
Cruciform DNA, a non-double helix form of DNA, can be generated as an intermediate in genetic recombination as well as from palindromic sequences under the effect of supercoiling. Eukaryotic cells are equipped with a DNA-binding protein that selectively recognizes cruciform DNA. Biochemical and immunological data showed that this protein is HMG1, an evolutionarily conserved, essential, and abundant component of the nucleus. The interaction with a ubiquitous protein points to a critical role for cruciform DNA conformations.
十字形DNA是一种非双螺旋形式的DNA，它可作为遗传重组的中间体产生，也可在超螺旋作用下由回文序列产生。真核细胞配备有一种能选择性识别十字形DNA的DNA结合蛋白。生化和免疫学数据表明，这种蛋白质是HMG1，它是细胞核中一种进化上保守、必不可少且含量丰富的成分。与一种普遍存在的蛋白质的相互作用表明十字形DNA构象具有关键作用。
