Majhi Sullip Kumar, Kumar Santosh
ICAR-National Bureau of Fish Genetic Resources, Division of Fish Conservation, Canal Ring Road, Dilkhusa P.O., Lucknow, Uttar Pradesh 226002, India.
Heliyon. 2017 Oct 31;3(10):e00434. doi: 10.1016/j.heliyon.2017.e00434. eCollection 2017 Oct.
The obligatory air-breathing catfish is a prime candidate for aquaculture owing to its unique taste, high growth rate, and hardy nature. However, recently the IUCN has listed the species under the endangered category because the population has critically declined in the wild. The sexually mature brooders are often collected from their natural habitats for seed production in captivity. In many cases, the brooder dies due to handling injuries or confinement stress. In this study, we demonstrated that viable progeny could be generated from freshly dead sexually mature . Three hours after death, the gonads were excised, macroscopically examined and gamete viability was evaluated. Artificial fertilization was performed by mixing the sperm suspension with the eggs. Water was added after 1 min of mixing to activate the fertilization process. We observed 85%-93% fertilization success from gametes derived from dead donors as opposed to 90%-95% from those derived from live control donors. The embryos showed normal development and resulted in the generation of 88%-92% viable progeny, which was similar to the progeny derived from control donors (92%-93%). The results obtained in this study will have profound implications in enhancing the seed production of endangered and could potentially be applied to other key commercially or endangered fish species.
由于其独特的味道、高生长率和耐寒性，须呼吸鲶鱼是水产养殖的主要候选对象。然而，最近国际自然保护联盟已将该物种列为濒危物种，因为其在野外的种群数量已急剧下降。性成熟的亲鱼常从其自然栖息地收集，用于圈养繁殖鱼苗。在许多情况下，亲鱼会因处理受伤或圈养压力而死亡。在本研究中，我们证明了可以从刚死亡的性成熟亲鱼中产生有活力的后代。死亡三小时后，切除性腺，进行宏观检查并评估配子活力。通过将精子悬浮液与卵子混合进行人工受精。混合1分钟后加水以激活受精过程。我们观察到，来自死亡供体的配子受精成功率为85%-93%，而来自活体对照供体的配子受精成功率为90%-95%。胚胎显示正常发育，并产生了88%-92%的有活力后代，这与来自对照供体的后代（92%-93%）相似。本研究获得的结果将对提高濒危鲶鱼的鱼苗产量具有深远意义，并有可能应用于其他重要的商业或濒危鱼类物种。
