Majhi Sullip Kumar, Maurya Pradip Kumar, Kumar Santosh, Mohindra Vindhya, Lal Kuldeep Kumar
ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, Dilkhusa P.O., Lucknow 226002, Uttar Pradesh, India.
MethodsX. 2020 May 23;7:100929. doi: 10.1016/j.mex.2020.100929. eCollection 2020.
Induced spawning is more inefficient in than in other fish species such as cyprinids and salmonids. Ovulation can be induced in the female by using pituitary extract or synthetic hormones. However, milt from the male cannot be obtained by hand stripping because the volume of seminal fluid in the testes is extremely low. Notably, similar problems are observed in other male catfishes such as and . Because milt from the males cannot be obtained for use in artificial fertilization of eggs, males are invariably sacrificed, and the testicular tissue is excised and macerated to obtain spermatozoa.•We developed an alternative approach that allowed harvesting of spermatozoa through surgery for artificial fertilization without sacrificing male fish.•The surgically obtained spermatozoa were used to inseminate eggs; the cross resulted in healthy progeny with a fertilization rate of 80%-98% and hatching of up to 85% of fertilized embryos; similar to those obtained using the conventional sacrificial approach (hatching percentage range of 45%-85%) [1].•This indicated the viability of partial surgical harvest of testicular tissue in seed production in for aquaculture without sacrificing male fish.
与鲤科鱼类和鲑科鱼类等其他鱼类相比，诱导产卵在[某种鱼类]中效率更低。可通过使用垂体提取物或合成激素诱导雌鱼排卵。然而，由于睾丸中的精液量极少，无法通过手工挤压从雄鱼获取精液。值得注意的是，在其他雄性鲶鱼如[其他鲶鱼种类]中也观察到类似问题。由于无法获取雄鱼精液用于卵子的人工授精，雄鱼总是被牺牲，切除并研磨睾丸组织以获取精子。•我们开发了一种替代方法，可通过手术采集[某种鱼类]精子用于人工授精，而无需牺牲雄鱼。•手术获取的精子用于使[某种鱼类]卵子受精；该杂交产生了健康的后代，受精率为80%-98%，受精胚胎的孵化率高达85%；与使用传统牺牲方法获得的结果相似（孵化率范围为45%-85%）[1]。•这表明在水产养殖的[某种鱼类]种子生产中，不牺牲雄鱼而部分手术采集睾丸组织是可行的。
