Cai Shu-Jyun, Li Ching-Wen, Weihs Daphne, Wang Gou-Jen
Graduate Institute of Biomedical Engineering, National Chung-Hsing University, Taichung, Taiwan.
Department of Mechanical Engineering, National Chung-Hsing University, Taichung, Taiwan.
Sci Technol Adv Mater. 2017 Dec 1;18(1):987-996. doi: 10.1080/14686996.2017.1406287. eCollection 2017.
The aim of this study was to develop a porous chitosan scaffold with long-acting drug release as an artificial dressing to promote skin wound healing. The dressing was fabricated by pre-freezing at different temperatures (-20 and -80 °C) for different periods of time, followed by freeze-drying to form porous chitosan scaffolds with different pore sizes. The chitosan scaffolds were then used to investigate the effect of the controlled release of fibroblast growth factor-basic (bFGF) and transforming growth factor-β1 (TGFβ1) on mouse fibroblast cells (L929) and bovine carotid endothelial cells (BEC). The biocompatibility of the prepared chitosan scaffold was confirmed with WST-1 proliferation and viability assay, which demonstrated that the material is suitable for cell growth. The results of this study show that the pore sizes of the porous scaffolds prepared by freeze-drying can change depending on the pre-freezing temperature and time via the formation of ice crystals. In this study, the scaffolds with the largest pore size were found to be 153 ± 32 μm and scaffolds with the smallest pores to be 34 ± 9 μm. Through cell culture analysis, it was found that the concentration that increased proliferation of L929 cells for bFGF was 0.005 to 0.1 ng/mL, and the concentration for TGFβ1 was 0.005 to 1 ng/mL. The cell culture of the chitosan scaffold and growth factors shows that 3.75 ng of bFGF in scaffolds with pore sizes of 153 ± 32 μm can promote L929 cell proliferation, while 400 pg of TGFβ1 in scaffolds with pore size of 34 ± 9 μm can enhance the proliferation of L929 cells, but also inhibit BEC proliferation. It is proposed that the prepared chitosan scaffolds can form a multi-drug (bFGF and TGFβ1) release dressing that has the ability to control wound healing via regulating the proliferation of different cell types.
本研究的目的是开发一种具有长效药物释放功能的多孔壳聚糖支架作为人工敷料，以促进皮肤伤口愈合。通过在不同温度（-20和-80°C）下预冷冻不同时间，然后冷冻干燥以形成具有不同孔径的多孔壳聚糖支架来制备该敷料。然后使用壳聚糖支架研究碱性成纤维细胞生长因子（bFGF）和转化生长因子-β1（TGFβ1）的控释对小鼠成纤维细胞（L929）和牛颈动脉内皮细胞（BEC）的影响。通过WST-1增殖和活力测定证实了所制备的壳聚糖支架的生物相容性，结果表明该材料适合细胞生长。本研究结果表明，通过冷冻干燥制备的多孔支架的孔径可根据预冷冻温度和时间通过冰晶的形成而改变。在本研究中，发现孔径最大的支架为153±32μm，孔径最小的支架为34±9μm。通过细胞培养分析发现，促进L929细胞增殖的bFGF浓度为0.005至0.1 ng/mL，TGFβ1的浓度为0.005至1 ng/mL。壳聚糖支架与生长因子的细胞培养表明，孔径为153±32μm的支架中3.75 ng的bFGF可促进L929细胞增殖，而孔径为34±9μm的支架中400 pg的TGFβ1可增强L929细胞的增殖，但也会抑制BEC增殖。有人提出，所制备的壳聚糖支架可以形成一种多药物（bFGF和TGFβ1）释放敷料，该敷料具有通过调节不同细胞类型的增殖来控制伤口愈合的能力。
