Du Xiaozheng, Yuan Bo, Wang Jinhai, Zhang Xinghua, Tian Liang, Ren Chaozhan, Zeng Huazong
Gansu University of CM, Lanzhou 730000, China.
the Second Hospital of Lanzhou University, Lanzhou 730000, China.
Zhongguo Zhen Jiu. 2017 Jan 12;37(1):55-60. doi: 10.13703/j.0255-2930.2017.01.014.
To compare the effects of different acupuncture methods on urine metabolites in rheumatoid arthritis (RA) rabbits, and to explore the specificity mechanism of heat-reinforcing acupuncture for RA.
A total of 40 clean purple-blue rabbits were randomly allocated to a normal group, a model group, a mild reinforcing-reducing needling (MRRN) group, a twirling-reinforcing needling (TRN) group and a heat-reinforcing needling (HRN) group, 8 rabbits in each one. Except the normal group, the rabbits in the remaining groups were treated with ovalbumin and freezing to establish RA model. The rabbits in the MRRN group, TRN group and HRN group were treated with MRRN, TRN and HRN at "Zusanli" (ST 36), respectively, 30 min per treatment, once a day for seven days. After treatment, 24-h urine was collected. The rabbits were sacrificed to collect synovial tissues of knee to perform morphology observation; the liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q/TOF-MS) was applied to measure urine metabolites. All the data were analyzed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA).
Compared with the normal group, the leucine-related metabolites, as main urine metabolites, were decreased in the model group (<0.05), while the purine-related metabolites and tryptophane-related metabolites were increased (<0.05). Compared with the model group, the leucine-related metabolites, as main urine metabolites, were increased in the three needling groups after treatment (<0.05), while the tryptophan-related metabolites andpurine-related metabolites were decreased (<0.05), moreover, the leucine-related metabolites in the HRN group were obviously higher than those in the MRRN group and TRN gruop (<0.05).
MRRN, TRN and HRN can regulate the pathway of leucine metabolism (energy metabolism), purine metabolism (oxidative damage) and tryptophane metabolism (immune regulation) for RA, The specificity of HRN for RA focuses on regulation of leucine metabolism (energy metabolism).
比较不同针刺方法对类风湿关节炎（RA）家兔尿液代谢产物的影响，探讨温针治疗RA的特异性机制。
将40只清洁级青紫蓝兔随机分为正常组、模型组、平补平泻针法组（MRRN组）、捻转补法组（TRN组）和温针组（HRN组），每组8只。除正常组外，其余各组家兔采用卵白蛋白和冷冻法建立RA模型。MRRN组、TRN组和HRN组家兔分别于“足三里”（ST 36）穴采用平补平泻针法、捻转补法和温针治疗，每次治疗30分钟，每天1次，连续7天。治疗后，收集24小时尿液。处死家兔，取膝关节滑膜组织进行形态学观察；采用液相色谱-四极杆飞行时间质谱（LC-Q/TOF-MS）测定尿液代谢产物。所有数据采用主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）进行分析。
与正常组比较，模型组作为主要尿液代谢产物的亮氨酸相关代谢产物减少（<0.05），而嘌呤相关代谢产物和色氨酸相关代谢产物增加（<0.05）。与模型组比较，3个针刺组治疗后作为主要尿液代谢产物的亮氨酸相关代谢产物增加（<0.05），而色氨酸相关代谢产物和嘌呤相关代谢产物减少（<0.05），且HRN组亮氨酸相关代谢产物明显高于MRRN组和TRN组（<0.05）。
平补平泻针法、捻转补法和温针可调节RA家兔的亮氨酸代谢途径（能量代谢）、嘌呤代谢途径（氧化损伤）和色氨酸代谢途径（免疫调节），温针治疗RA的特异性集中在对亮氨酸代谢途径（能量代谢）的调节上。
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