Yuyama Kamila Tomoko, Chepkirui Clara, Wendt Lucile, Fortkamp Diana, Stadler Marc, Abraham Wolf-Rainer
Chemical Microbiology, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, Germany.
Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, Germany.
Microorganisms. 2017 Dec 12;5(4):80. doi: 10.3390/microorganisms5040080.
Treating infections organized in biofilms is a challenge due to the resistance of the pathogens against antibiotics and host immune cells. Many fungi grow in a wet environment, favorable for the growth of bacterial biofilms, and we speculated that fungi possess some strategies to control these bacterial biofilms A fungus identified as , was collected in the Harz Mountains, Germany, and its mycelial culture was fermented in different culture media for 67 days to test its biological potential against bacterial biofilms. Sclerin, sclerin diacid and its 3-methyl monoester (methyl 1-(5-hydroxy-6-carboxylic-2,3,4-trimethylphenyl) propionate) are here described for the first time from this fungus. Sclerin and its diacid interfered with the biofilm formation of the pathogen , inhibiting 86% and 80% of the biofilm at 256 μg mL, respectively, but not killing the bacterium. Interestingly, the monomethylester of sclerin diacid was inactive. Although these compounds did not possess any activity against a pre-formed biofilm, they prevented its formation at subtoxic concentrations. Furthermore, sclerin and its diacid displayed a high specificity against , indicating a good strategy against pathogenic biofilms when combined with antibiotics.
由于病原体对抗生素和宿主免疫细胞具有抗性，因此治疗由生物膜组织的感染是一项挑战。许多真菌生长在潮湿的环境中，有利于细菌生物膜的生长，我们推测真菌拥有一些控制这些细菌生物膜的策略。一种被鉴定为 的真菌是在德国哈茨山脉采集的，其菌丝体培养物在不同培养基中发酵67天，以测试其对细菌生物膜的生物活性。本文首次描述了从这种真菌中提取的硬脂菌素、硬脂菌素二酸及其3-甲基单酯（1-(5-羟基-6-羧基-2,3,4-三甲基苯基)丙酸甲酯）。硬脂菌素及其二酸干扰了病原体的生物膜形成，在256μg/mL时分别抑制了86%和80%的生物膜形成，但没有杀死细菌。有趣的是，硬脂菌素二酸的单甲酯没有活性。尽管这些化合物对预先形成的生物膜没有任何活性，但它们在亚毒性浓度下阻止了生物膜的形成。此外，硬脂菌素及其二酸对 表现出高度特异性，表明与抗生素联合使用时是对抗致病性生物膜的良好策略。
