Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand.
Department of Microbial Drugs, Helmholtz Centre for Infection Research and German Centre for Infection Research (DZIF), partner site Hannover/Braunschweig, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
Molecules. 2018 Aug 30;23(9):2190. doi: 10.3390/molecules23092190.
species are well recorded from both monocotyledons and dicotyledons. As part of a research program to discover biologically active compounds from plant-associated Dothideomycetes in Thailand, the strain sp. (MFLUCC 17-2059), which represents an undescribed species, was isolated from Kurz, fermented in yeast-malt medium and explored for its secondary metabolite production. Bioassay-guided fractionation of the crude extract yielded the new abscisic acid derivative, roussoellenic acid (), along with pestabacillin B (), a related congener, and the cyclodipeptide, (-Pro--Ile) (). The structure of was determined by 2D NMR spectroscopy and HR-ESIMS data analysis. Compounds and showed inhibitory activity on biofilm formation by . The biofilm formation of was reduced to 34% at 16 µg/mL by roussoellenic acid (), while pestabacillin B () only showed 36% inhibition at 256 µg/mL. In addition, compound also had weak cytotoxic effects on L929 murine fibroblasts and human KB3-1 cancer cells.
该物种在单子叶植物和双子叶植物中均有很好的记录。作为在泰国从植物相关的腔菌纲中发现生物活性化合物的研究计划的一部分，分离到了代表未描述物种的菌株 sp. (MFLUCC 17-2059)，其从 Kurz 发酵在酵母-麦芽培养基中，并探索其次生代谢产物的产生。根据生物活性导向的粗提物进行的分离得到了新的脱落酸衍生物 Roussoellenic acid ()，以及相关的同系物 Pestabacillin B ()和环二肽 (-Pro--Ile) ()。通过二维 NMR 光谱和 HR-ESIMS 数据分析确定了 Roussoellenic acid ()的结构。化合物 和 对 的生物膜形成表现出抑制活性。 Roussoellenic acid ()在 16 µg/mL 时将生物膜形成降低到 34%，而 Pestabacillin B ()在 256 µg/mL 时仅表现出 36%的抑制作用。此外，化合物 对 L929 小鼠成纤维细胞和人 KB3-1 癌细胞也表现出较弱的细胞毒性作用。
