Arnauld E, Bibene V, Meynard J, Rodriguez F, Vincent J D
Institut National de la Santé et de la Recherche Médicale, U.176, Bordeaux, France.
Am J Physiol. 1989 Mar;256(3 Pt 2):R674-84. doi: 10.1152/ajpregu.1989.256.3.R674.
The effect of arginine vasopressin (AVP) on the duration and the relative proportion of sleeping and wakeful periods has been investigated. Vigilance states were determined by visual scoring of polygraphic recordings from unrestrained rats. Animals were implanted with a cannula into the third ventricle through which AVP or related drugs, dissolved in artificial cerebrospinal fluid, were infused at a constant rate by an osmotic pump. Polygraphic data were collected 24 h/day from day 4 to day 9. Recordings were continued for 3 additional days during AVP recovery. AVP infusions significantly increased the amount of time spent in waking compared with control or recovery periods (12%). This effect was mimicked by an AVP agonist (2-phenylalanine, 8-ornithine oxytocin). Oxytocin, a peptide structurally close to AVP, induced a mild change in waking time. The infusion of an AVP antagonist, 1-desaminopenicillamine-2-(O-methyl)tyrosine-arginine vasopressin (dPTyr(Me)AVP), or of anti-AVP antibodies significantly decreased duration of waking. The infusion of antioxytocin antibodies did not modify the duration of waking. The effects of structural analogues of AVP relatively specific for each type of peripheral AVP receptor indicated the participation of a V1-like AVP receptor in the action of AVP on waking time. During infusion of anti-AVP antibodies and dPTyr(Me)AVP and during the first days of recovery from AVP infusion, the ultradian rhythmic distribution of sleep and wakefulness was still present, but the amplitude of the circadian rhythm was reduced.
已对精氨酸加压素（AVP）对睡眠时间和清醒时间的持续时长及相对比例的影响进行了研究。通过对未束缚大鼠的多导记录进行视觉评分来确定警觉状态。将套管植入动物的第三脑室，通过该套管，以恒定速率由渗透泵输注溶解于人工脑脊液中的AVP或相关药物。从第4天至第9天，每天24小时收集多导数据。在AVP恢复期间，记录再持续3天。与对照期或恢复期相比，输注AVP显著增加了清醒时间（12%）。一种AVP激动剂（2-苯丙氨酸，8-鸟氨酸催产素）模拟了这种效应。催产素是一种在结构上与AVP相近的肽，可引起清醒时间的轻微变化。输注AVP拮抗剂1-脱氨基青霉胺-2-（O-甲基）酪氨酸-精氨酸加压素（dPTyr(Me)AVP）或抗AVP抗体可显著缩短清醒时间。输注抗催产素抗体并未改变清醒时间。AVP的结构类似物对每种外周AVP受体类型具有相对特异性，其作用表明一种类V1型AVP受体参与了AVP对清醒时间的作用。在输注抗AVP抗体和dPTyr(Me)AVP期间以及从AVP输注恢复的最初几天，睡眠和清醒的超日节律分布仍然存在，但昼夜节律的幅度减小。
