Wallingford R A, Ewing A G
Anal Chem. 1989 Jan 15;61(2):98-100. doi: 10.1021/ac00177a004.
Capillary zone electrophoresis with electrochemical detection is demonstrated with columns having only 9-microns inner diameter. Amperometric detection limits of 0.7 amol are reported for serotonin. The difficult problem of resolving serotonin and dopamine--two neurotransmitters of interest having similar electrophoretic mobilities--is addressed by chemical means to improve selectivity. These include buffer modification with 2-propanol and a system employing borate complexation of the catechol in combination with sodium dodecyl sulfate micelles.
内径仅9微米的色谱柱用于演示毛细管区带电泳结合电化学检测。血清素的安培检测限为0.7阿托摩尔。血清素和多巴胺是两种具有相似电泳迁移率的重要神经递质，解决它们之间分离的难题可采用化学方法来提高选择性。这些方法包括用异丙醇改性缓冲液，以及一种将儿茶酚硼酸盐络合与十二烷基硫酸钠胶束相结合的体系。
