Functional Unit for Research into Chronic Diseases (UFIEC), Institute of Health Carlos III, 28220 Madrid, Spain.
Spanish National Center for Microbiology (CNM), Institute of Health Carlos III, 28220 Madrid, Spain.
Viruses. 2017 Dec 10;9(12):379. doi: 10.3390/v9120379.
Human respiratory syncytial virus (HRSV) is a major cause of serious pediatric respiratory diseases that lacks effective vaccine or specific therapeutics. Although our understanding about HRSV biology has dramatically increased during the last decades, the need for adequate models of HRSV infection is compelling. We have generated a two-dimensional minilung from human embryonic stem cells (hESCs). The differentiation protocol yielded at least six types of lung and airway cells, although it is biased toward the generation of distal cells. We show evidence of HRSV replication in lung cells, and the induction of innate and proinflammatory responses, thus supporting its use as a model for the study of HRSV-host interactions.
人呼吸道合胞病毒（HRSV）是一种主要的儿童呼吸道疾病病原体，目前尚无有效的疫苗或特效疗法。尽管我们对 HRSV 生物学的理解在过去几十年中取得了显著进展，但仍迫切需要适当的 HRSV 感染模型。我们已经从人胚胎干细胞（hESCs）中生成了二维迷你肺。该分化方案产生了至少六种类型的肺和气道细胞，但偏向于生成远端细胞。我们证明了 HRSV 在肺细胞中的复制，以及先天和促炎反应的诱导，因此支持将其用作研究 HRSV-宿主相互作用的模型。
