Functional Unit for Research into Chronic Diseases, Institute of Health Carlos III, Ctra. Majadahonda-Pozuelo Km 2, 28220, Madrid, Spain.
Stem Cell Res Ther. 2018 Jul 11;9(1):186. doi: 10.1186/s13287-018-0943-9.
Organoids from human pluripotent stem cells are becoming suitable models for studies of organ development, drug screening, regenerative medicine, and disease modeling. Three-dimensional minilungs in Matrigel culture have recently been generated from human embryonic stem cells. These particular organoids, named lung bud organoids, showed branching airway and early alveolar structures resembling those present in lungs from the second trimester of human gestation. We show here that the treatment of such organoids with a lung and airway epithelial maturation cocktail containing dexamethasone drives lung bud organoids to the formation of paddle-racquet like structures. This strategy may help to increase the versatility of lung organoids and to generate structures more advanced than the original branching texture.
人多能干细胞来源的类器官正在成为研究器官发育、药物筛选、再生医学和疾病建模的合适模型。最近已经从人类胚胎干细胞中生成了 Matrigel 培养中的三维迷你肺。这些特定的类器官，称为肺芽类器官，显示出分支气道和早期肺泡结构，类似于人类妊娠第二个三个月的肺部。我们在这里表明，用含有地塞米松的肺和气道上皮成熟鸡尾酒处理此类类器官可促使肺芽类器官形成桨球拍样结构。这种策略可能有助于增加肺类器官的多功能性，并生成比原始分支结构更先进的结构。
