Li Yuan-Sheng, Chen Pao-Jen, Wu Li-Wei, Chou Pei-Wen, Sun Li-Yi, Chiou Tzyy-Wen
Department of Life Science and Graduate Institute of Biotechnology, National Dong Hwa University, No. 1, Sec. 2, Da Hsueh Rd., Shou-Feng, Hualien, Taiwan, ROC.
Department of Obstetrics and Gynecology, Ching-Kuo Campus of Min-Sheng Hospital, Taoyuan, Taiwan, ROC.
Cytotechnology. 2018 Feb;70(1):55-66. doi: 10.1007/s10616-017-0162-8. Epub 2017 Dec 12.
The success of stem cell application in regenerative medicine, usually require a stable source of stem or progenitor cells. Fat tissue represents a good source of stem cells because it is rich in stem cells and there are fewer ethical issues related to the use of such stem cells, unlike embryonic stem cells. Therefore, there has been increased interest in adipose-derived stem cells (ADSCs) for tissue engineering applications. Here, we aim to provide an easy processing method for isolating adult stem cells from human adipose tissue harvested from the subcutaneous fat of the abdominal wall during gynecologic surgery. We used a homogenizer to mince fat and compared the results with those obtained from the traditional cut method involving a sterile scalpel and forceps. Our results showed that our method provides another stable and quality source of stem cells that could be used in cases with a large quantity of fat. Furthermore, we found that pregnancy adipose-derived stem cells (P-ADSCs) could be maintained in vitro for extended periods with a stable population doubling and low senescence levels. P-ADSCs could also differentiate in vitro into adipogenic, osteogenic, chondrogenic, and insulin-producing cells in the presence of lineage-specific induction factors. In conclusion, like human lipoaspirates, adipose tissues obtained from pregnant women contain multipotent cells with better proliferation and showed great promise for use in both stem cell banking studies as well as in stem cell therapy.
干细胞在再生医学中的应用成功通常需要稳定的干细胞或祖细胞来源。脂肪组织是干细胞的良好来源，因为它富含干细胞，且与胚胎干细胞不同，使用此类干细胞涉及的伦理问题较少。因此，脂肪来源干细胞（ADSCs）在组织工程应用中的关注度日益增加。在此，我们旨在提供一种简便的处理方法，用于从妇科手术中获取的腹壁皮下脂肪组织中分离成人干细胞。我们使用匀浆器切碎脂肪，并将结果与传统的使用无菌手术刀和镊子的切割方法所得结果进行比较。我们的结果表明，我们的方法提供了另一种稳定且优质的干细胞来源，可用于脂肪量较大的情况。此外，我们发现妊娠脂肪来源干细胞（P - ADSCs）可在体外长时间维持，群体倍增稳定且衰老水平低。在存在谱系特异性诱导因子的情况下，P - ADSCs还可在体外分化为脂肪生成细胞、成骨细胞、软骨生成细胞和胰岛素生成细胞。总之，与人类抽脂物一样，从孕妇获取的脂肪组织含有具有更好增殖能力的多能细胞，在干细胞库研究以及干细胞治疗中都显示出巨大的应用前景。
