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BEAMing灯：用于血浆中丙型肝炎病毒数字检测的单分子捕获和珠上等温扩增

BEAMing LAMP: single-molecule capture and on-bead isothermal amplification for digital detection of hepatitis C virus in plasma.

作者信息

Chen Jiyun, Xu Xiaomin, Huang Zhimei, Luo Yuan, Tang Lijuan, Jiang Jian-Hui

机构信息

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.

出版信息

Chem Commun (Camb). 2018 Jan 2;54(3):291-294. doi: 10.1039/c7cc08403j.

DOI:10.1039/c7cc08403j
PMID:29235591
Abstract

A novel dNAD platform (BEAMing LAMP) by combining emulsion micro-reactors, single-molecule magnetic capture and on-bead loop-mediated isothermal amplification has been developed for DNA detection, which enables absolute and high-precision quantification of a target with a detection limit of 300 copies.

摘要

通过结合乳液微反应器、单分子磁捕获和磁珠上的环介导等温扩增，开发了一种新型的数字核酸检测平台（BEAMing LAMP）用于DNA检测，该平台能够对目标进行绝对且高精度的定量，检测限为300拷贝。

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