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利用虹鳟幼鱼的运动活性快速检测亚致死毒性

Rapid Detection of Sublethal Toxicity Using Locomotor Activity of Rainbow Trout Juveniles.

作者信息

Makaras Tomas, Svecevičius Gintaras, Kazlauskienė Nijolė, Montvydienė Danguolė

机构信息

Laboratory of Ecology and Physiology of Hydrobionts of Nature Research Centre, Akademijos St. 2, 08412, Vilnius, Lithuania.

出版信息

Bull Environ Contam Toxicol. 2018 Feb;100(2):221-227. doi: 10.1007/s00128-017-2244-x. Epub 2017 Dec 13.

Abstract

This study examined changes in locomotor activity of the rainbow trout (Oncorhynchus mykiss) juveniles exposed to sublethal concentrations of hexavalent chromium (Cr) (as a single pollutant) and landfill leachate (as an complex mixture of mainly organic compounds, including trace amounts of metals). Fish were first examined for baseline behavior patterns for 3 h to determine the appropriate duration of acclimation and the control level. The average velocity of fish was found to be the most informative among other endpoints throughout the 3 h long acclimation. Under the effect of both test substances, fish locomotor activity significantly increased after 5 min reaching maximum values after 10 min of exposure. The juveniles exposed to leachate were more responsive than those exposed to Cr. However, in this study we did not succeed in identifying pollutants from the elicited fish behavioral response patterns.

摘要

本研究考察了暴露于亚致死浓度六价铬(Cr)(作为单一污染物)和垃圾渗滤液(作为主要有机化合物的复杂混合物,包括痕量金属)的虹鳟(Oncorhynchus mykiss)幼鱼的运动活性变化。首先对鱼类进行3小时的基线行为模式检查,以确定合适的驯化持续时间和对照水平。在长达3小时的驯化过程中,发现鱼类的平均速度在其他终点指标中最具信息量。在两种受试物质的作用下,鱼类运动活性在5分钟后显著增加,暴露10分钟后达到最大值。暴露于渗滤液的幼鱼比暴露于Cr的幼鱼反应更灵敏。然而,在本研究中,我们未能从引发的鱼类行为反应模式中识别出污染物。

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