工程改造基因组精简的枯草芽孢杆菌以利用木糖生产3-羟基丁酮
Engineering genome-reduced Bacillus subtilis for acetoin production from xylose.
作者信息
Yan Panpan, Wu Yuanqing, Yang Li, Wang Zhiwen, Chen Tao
机构信息
Key Laboratory of Systems Bioengineering (Ministry of Education), SynBio Research Platform, Collaborative Innovation Center for Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
College of life Science, Shihezi University, Shihezi, 832000, People's Republic of China.
出版信息
Biotechnol Lett. 2018 Feb;40(2):393-398. doi: 10.1007/s10529-017-2481-4. Epub 2017 Dec 13.
OBJECTIVES
To investigate the capacity of a genome-reduced Bacillus subtilis strain as chassis cell for acetoin production from xylose.
RESULTS
To endow the genome-reduced Bacillus subtilis strain BSK814 with the ability to utilize xylose, we inserted a native xyl operon into its genome and deleted the araR gene. The resulting strain BSK814A2 produced 2.94 g acetoin/l from 10 g xylose/l, which was 39% higher than control strain BSK19A2. The deletion of the bdhA and acoA genes further improved xylose utilization efficiency and increased acetoin production to 3.71 g/l in BSK814A4. Finally, BSK814A4 produced up to 23.3 g acetoin/l from 50 g xylose/l, with a yield of 0.46 g/g xylose. Both the titer and yield were 39% higher than those of control strain BSK19A4.
CONCLUSIONS
As a chassis cell, genome-reduced B. subtilis showed significantly improved capacity for the production of the overflow product acetoin from xylose compared with wild-type strain.
目的
研究基因组精简的枯草芽孢杆菌菌株作为底盘细胞从木糖生产3-羟基丁酮的能力。
结果
为了使基因组精简的枯草芽孢杆菌菌株BSK814具备利用木糖的能力，我们将一个天然木糖操纵子插入其基因组并删除了araR基因。所得菌株BSK814A2以10 g/l木糖为原料生产了2.94 g/l 3-羟基丁酮，比对照菌株BSK19A2高39%。删除bdhA和acoA基因进一步提高了木糖利用效率，并使BSK814A4中的3-羟基丁酮产量增加至3.71 g/l。最终，BSK814A4以50 g/l木糖为原料生产了高达23.3 g/l 3-羟基丁酮，产率为0.46 g/g木糖。其产量和产率均比对照菌株BSK19A4高39%。
结论
作为底盘细胞，与野生型菌株相比，基因组精简的枯草芽孢杆菌从木糖生产溢流产物3-羟基丁酮的能力显著提高。
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