Brucka Marta, Sheberstov Kirill F, Jeannerat Damien
Department of Organic Chemistry, University of Geneva, 30 Quai E. Ansermet, 1211, Geneva 4, Switzerland.
State Scientific Research Institute of Chemistry and Technology of Organoelement Compounds, 38 Shosse Entuziastov, 105118, Moscow, Russia.
Magn Reson Chem. 2018 Oct;56(10):1021-1028. doi: 10.1002/mrc.4697. Epub 2018 Jan 12.
The two most compelling methods for broadband homonuclear decoupling currently available, Zangger-Sterk (ZS) and pure shift yielded by chirp excitation (PSYCHE), were successfully adapted and tested on the C isotope. When applied during the indirect carbon evolution in the HSQC experiment, they both entirely eliminated the extended carbon-carbon multiplet structures observed in this dimension of a non-decoupled HSQC spectrum of C-enriched cholesterol. The optimized selective pulse modulated using novel non-equidistant scheme for multisite refocusing (ZS) and the small flip angle saltire chirps (PSYCHE) both proved to be robust and efficient in providing decoupled spectra with a sensitivity of about 25% that of the non-decoupled HSQC spectra with improved quality compared to earlier results.
目前可用的两种最具说服力的宽带同核去耦方法，即赞格-斯特克（ZS）法和线性调频激励产生的纯位移法（PSYCHE），已成功地应用于碳同位素并进行了测试。当在HSQC实验的间接碳演化过程中应用时，它们都完全消除了在富含碳的胆固醇的非去耦HSQC谱的这一维度中观察到的扩展碳-碳多重峰结构。使用用于多部位重聚焦的新型非等距方案调制的优化选择性脉冲（ZS）和小翻转角十字形线性调频脉冲（PSYCHE），在提供去耦谱方面都被证明是稳健且有效的，其灵敏度约为非去耦HSQC谱的25%，与早期结果相比，质量有所提高。
