对临床分离株分枝杆菌属MHSD3中一种II型毒素-抗毒素系统的初步见解，该系统类似于ε/ζ系统。
First insights into a type II toxin-antitoxin system from the clinical isolate Mycobacterium sp. MHSD3, similar to epsilon/zeta systems.
作者信息
Jaén-Luchoro Daniel, Aliaga-Lozano Francisco, Gomila Rosa Maria, Gomila Margarita, Salvà-Serra Francisco, Lalucat Jorge, Bennasar-Figueras Antoni
机构信息
Microbiologia, Departament de Biologia, Universitat de les Illes Balears, Palma de Mallorca, Spain.
Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
出版信息
PLoS One. 2017 Dec 13;12(12):e0189459. doi: 10.1371/journal.pone.0189459. eCollection 2017.
A putative type II toxin-antitoxin (TA) system was found in the clinical isolate Mycobacterium sp. MHSD3, a strain closely related to Mycobacterium chelonae. Further analyses of the protein sequences of the two genes revealed the presence of domains related to a TA system. BLAST analyses indicated the presence of closely related proteins in the genomes of other recently published M. chelonae strains. The functionality of both elements of the TA system was demonstrated when expressed in Escherichia coli cells, and the predicted structure of the toxin is very similar to those of well-known zeta-toxins, leading to the definition of a type II TA system similar to epsilon/zeta TA systems in strains that are closely related to M. chelonae.
在临床分离株龟分枝杆菌密切相关的菌株Mycobacterium sp. MHSD3中发现了一个假定的II型毒素-抗毒素（TA）系统。对这两个基因的蛋白质序列进行进一步分析，发现了与TA系统相关的结构域。BLAST分析表明，在其他最近发表的龟分枝杆菌菌株的基因组中存在密切相关的蛋白质。当在大肠杆菌细胞中表达时，证明了TA系统两个元件的功能，并且毒素的预测结构与著名的ζ毒素非常相似，从而在与龟分枝杆菌密切相关的菌株中定义了一种类似于ε/ζ TA系统的II型TA系统。
Zotero 插件