Guimarães Ana Paula Franttini Garcia Moreno, Butezloff Mariana Maloste, Zamarioli Ariane, Issa João Paulo Mardegan, Volpon José Batista
Fellow Master degree, Postgraduate Program in Health Sciences Applied to the Locomotor System, School of Medicine, Universidade de São Paulo (USP), Ribeirao Preto-SP, Brazil. Design of the study, technical procedures, acquisition and interpretation of data, manuscript preparation.
Fellow PhD degree, Postgraduate Program in Health Sciences Applied to the Locomotor System, School of Medicine, USP, Ribeirao Preto-SP, Brazil. Technical procedures, acquisition of data.
Acta Cir Bras. 2017 Nov;32(11):924-934. doi: 10.1590/s0102-865020170110000004.
To evaluate the influence of nandrolone decanoate on fracture healing and bone quality in normal rats.
Male rats were assigned to four groups (n=28/group): Control group consisting of animals without any intervention, Nandrolone decanoate (DN) group consisting of animals that received intramuscular injection of nandrolone decanoate, Fracture group consisting of animals with a fracture at the mid-diaphysis of the femur, and Fracture and nandrolone decanoate group consisting of animals with a femur fracture and treatment with nandrolone decanoate. Fractures were created at the mid-diaphysis of the right femur by a blunt trauma and internally fixed using an intramedullary steel wire. The DN was injected intramuscularly twice per week (10 mg/kg of body mass). The femurs were measured and evaluated by densitometry and mechanical resistance after animal euthanasia. The newly formed bone and collagen type I levels were quantified in the callus.
The treated animals had longer femurs after 28 days. The quality of the intact bone was not significantly different between groups. The bone callus did show a larger mass in the treated rats.
The administration of nandrolone decanoate did not affect the quality of the intact bone, but might have enhanced the bone callus formation.
评估癸酸诺龙对正常大鼠骨折愈合及骨质量的影响。
将雄性大鼠分为四组（每组n = 28）：对照组为未接受任何干预的动物；癸酸诺龙（DN）组为接受癸酸诺龙肌肉注射的动物；骨折组为股骨中骨干发生骨折的动物；骨折加癸酸诺龙组为股骨骨折并接受癸酸诺龙治疗的动物。通过钝性创伤造成右侧股骨中骨干骨折，并用髓内钢丝进行内固定。每周两次肌肉注射DN（10 mg/kg体重）。动物安乐死后，通过骨密度测定和力学抗性对股骨进行测量和评估。对骨痂中新生骨和I型胶原水平进行定量分析。
28天后，接受治疗的动物股骨更长。各组间完整骨的质量无显著差异。治疗组大鼠的骨痂质量确实更大。
癸酸诺龙给药未影响完整骨的质量，但可能促进了骨痂形成。
